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当前位置: 首页 > 文章 > 白羽王鸽卵清蛋白关联蛋白Y基因(OVALY)克隆与生物信息学分析 浙江农业学报 2019 (5) 704-708
Position: Home > Articles > 白羽王鸽卵清蛋白关联蛋白Y基因(OVALY)克隆与生物信息学分析 Acta Agriculturae Zhejiangensis 2019 (5) 704-708

白羽王鸽卵清蛋白关联蛋白Y基因(OVALY)克隆与生物信息学分析

作  者：
徐小钦;杜雪;李国勤;张龙;陶争荣;卢立志
单  位：
浙江省农业科学院畜牧兽医研究所
关键词：
白羽王鸽;卵清蛋白关联蛋白Y(OVALY);RACE
摘  要：
旨在获得白羽王鸽(Columba livia)卵清蛋白关联蛋白Y基因(OVALY)的cDNA序列,并进行生物信息分析.研究结果显示:OVALY基因cDNA全长为2 068 bp,其中5′非编码区序列180 bp,3′非编码区序列720 bp,开放阅读框1 164 bp,编码的蛋白分子量约44.19 ku,含氨基酸残基388个(GenBank∶KX230792);与NCBI数据库中朱鹮(Nipponianippon)OVALY基因序列同源性最高(81%),与白尾鹰(Haliaeetusalbicilla)和沙鸡(Pteroclesgutturalis)OVALY基因序列的同源性分别为80%和79%.经潜在糖基化位点和磷酸化位点预测分析发现,OVALY蛋白结构中潜在糖基化位点和磷酸化位点分别为4个和22个;经CDD(conserved domain database)数据库分析发现,OVALY蛋白具有丝氨酸蛋白酶抑制剂家族的反应中心区域,属于丝氨酸蛋白酶抑制剂超家族.
作  者：
XU Xiaoqin;DU Xue;LI Guoqin;ZHANG Long;TAO Zhengrong;LU Lizhi;Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences;Institute of Ecology, China West Normal University;College of Animal Sciences & Technology, Nanjing Agricultural University;Key Laboratory of Information Traceability for Agricultural Products, Ministry of Agriculture and Rural Affairs;
单  位：
XU Xiaoqin%DU Xue%LI Guoqin%ZHANG Long%TAO Zhengrong%LU Lizhi%Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences%Institute of Ecology, China West Normal University%College of Animal Sciences & Technology, Nanjing Agricultural University%Key Laboratory of Information Traceability for Agricultural Products, Ministry of Agriculture and Rural Affairs
关键词：
Columba livia;;OVALY;;RACE
摘  要：
The experiment was conducted to clone the whole cDNA of OVALY gene in Columba liviaand analyze the bioinformatic information of the cDNA sequence.The nucleotide sequence of a full-length 2 068 bp cDNA for OVALY was obtained, containing 5′UTR(180 bp), CDS(1 164 bp) and 3′UTR(720 bp), and coding 388 amino acids(44.19 ku, GenBank accession number: KX230792). The OVALY cDNA sequence of white king pigeon was most similar to Nipponianippon, and the homology was 81%. This sequence was also similar to Haliaeetusalbicilla and Pteroclesgutturalis, and the homologies were 80% and 79%, respectively. 4 N-glycosylated sites and 22 phosphorylation sites were detected in these amino acids. Conserved domain analysis of OVALY protein showed this protein had a conserved domain for serpin superfamily.

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