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当前位置: 首页 > 文章 > 蛋鸡肠道嗜酸乳杆菌的分离鉴定及益生特性研究 畜牧与饲料科学 2015,36 (8) 22-25
Position: Home > Articles > Identification and Probiotic Properties Analysis of Lactobacillus acidophilus Isolated from Intestinal Tract of Layer Animal Husbandry and Feed Science 2015,36 (8) 22-25

蛋鸡肠道嗜酸乳杆菌的分离鉴定及益生特性研究

作  者：
LI Chunho
单  位：
Anqiu Animal Husbandry Bureau of Shandong Provinc
关键词：
isolate;probiotic properties;preferable;lactobacillus acidophilus;selected;incubate
摘  要：
为筛选具有益生特性的嗜酸乳杆菌,采用常规方法从山东省潍坊市及周边地区蛋鸡肠道内容物样本中分离乳酸菌。通过测定发酵液p H值,筛选产酸性能较好的菌株;采用PCR及测序技术对筛选到的菌株进行分子生物学鉴定;采用常规方法评价筛选到的菌株的益生特性。在分离到的9株乳酸菌中,筛选到1株产酸性能较好的菌株(命名为XMR-3),分子生物学鉴定结果显示,该菌株为嗜酸乳杆菌(Lactobacillus acidophilus)。该菌株在培养6 h之后进入对数生长期,至16 h时,活菌数最高,此时培养液p H值为3.92;在p H值为3.0和4.0的酸性环境中培养4 h后,存活率均在99%以上;在0.3%的胆盐环境中,存活率为87.3%;对4种常见致病菌的抑菌圈直径均在20 mm以上。筛选到的菌株表现出一定的益生特性,在畜禽微生态制剂产品开发方面具有应用潜力。
译  名：
Identification and Probiotic Properties Analysis of Lactobacillus acidophilus Isolated from Intestinal Tract of Layer
作  者：
LI Chun-hong;Anqiu Animal Husbandry Bureau of Shandong Province;
关键词：
layer;;Lactobacillus acidophilus;;isolation;;identification;;phylogenetic analysis;;probiotics
摘  要：
In order to screen Lactobacillus acidophilus with probiotic properties, the intestinal contents samples collected from Weifang and surrounding area of Shandong Province were used to isolate the Lactobacillus strains by conventional method. The isolates with preferable acid production performance were selected by determining the p H value of fermentation liquid. The screened isolates were molecularly identified by using PCR assay and sequencing. The probiotic properties of the selected isolates were evaluated by conventional method. Among the 9 isolated strains, one isolate with preferable acid production performance,named as XMR-3, was found and was molecularly identified as Lactobacillus acidophilus. The isolate got into the logarithmic growth phase after six-hour incubation. The live bacteria number reached the peak when it was incubated for 16 h, and the p H value of its culture fluid was 3.92. The survival rate of the isolate was above 99% when it was incubated in broth with p H value of3.0 and 4.0, and that of the isolate was 87.3% when it was incubated in broth with 0.3% bile salt. The antibacterial circle diameters against common pathogenic bacteria were all above 20 mm. The selected Lactobacillus acidophilus isolate showed preferable probiotic properties and had potentiality in the development of probiotics products for animal use.

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