当前位置: 首页 > 文章 > 环介导等温扩增技术快速检测志贺氏菌的研究 安徽农业科学 2011,39 (14) 13-15+62
Position: Home > Articles > Research on the Detection of Shigella by LAMP Journal of Anhui Agricultural Sciences 2011,39 (14) 13-15+62

环介导等温扩增技术快速检测志贺氏菌的研究

作  者:
马晓燕;张会彦;宋明明;张先舟;王羽;张伟
单  位:
河北农业大学食品科技学院
关键词:
环介导等温扩增技术;检测;志贺氏菌
摘  要:
[目的]利用环介导等温扩增技术快速检测志贺氏菌。[方法]以志贺氏菌侵袭性质粒抗原H基因(ipaH)作为靶序列,设计引物,优化Mg2+浓度、Bst酶浓度等反应条件,建立LAMP反应体系。[结果]确定了环介导等温扩增技术检测志贺氏菌的适宜反应条件;该法检测志贺氏菌的灵敏度为62 cfu/ml。[结论]该研究初步建立了一种利用LAMP快速检测志贺氏菌的方法,为食品中志贺氏菌快速检测构建了一个技术平台。
译  名:
Research on the Detection of Shigella by LAMP
作  者:
MA Xiao-yan et al(Colloge of Food Science & Technology,Hebei Agriculture University,Baoding,Hebei 071001)
关键词:
LAMP;Detection;Shigella
摘  要:
[Objective] The aim was to detect Shigella by using the loop-mediated isothermal amplification(LAMP) technology.[Method] The sequences of ipaH of Shigella was used as target sequences to design primers,the reaction conditions such as Mg2+ concentration,Bst DNA polymerase concentration were optimized for establishing LAMP system.[Result] The reaction conditions were optimized and the sensitivity of LAMP assay was 62 cfu/ml.[Conclusion] LAMP technology for detection of Shigella was developed,and a technology platform was constructed for the rapid detection of shigella in food.

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