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当前位置: 首页 > 文章 > HPLC-ELSD法同时测定食品中的10种糖和糖醇 食品科学 2013,34 (12) 286-291
Position: Home > Articles > Simultaneous Determination by HPLC-ELSD of Ten Kinds of Sugars and Sugar Alcohols in Foods FOOD SCIENCE 2013,34 (12) 286-291

HPLC-ELSD法同时测定食品中的10种糖和糖醇

作  者：
林慧;颜春荣;徐春祥;蔡晶;王多娇
单  位：
江苏省产品质量监督检验研究院
关键词：
高效液相色谱法(HPLC);蒸发光散射检测(ELSD);食品;糖;糖醇
摘  要：
建立高效液相色谱-蒸发光散射法(HPLC-ELSD)同时测定食品中果糖、葡萄糖、蔗糖、麦芽糖和乳糖、赤藓糖醇、木糖醇、山梨糖醇、甘露糖醇、麦芽糖醇的方法。方法采用Grace Previl Carbohydrate ES色谱柱,柱温30℃;乙腈-水为流动相梯度洗脱,流速1.0mL/min;蒸发光散射检测器,飘移管温度95℃,氮气流量2.5L/min。结果表明:5种糖和5种糖醇均在线性0.2～20mg/mL范围内,线性良好,精密度RSD小于5%,回收率91.53%～105.2%。本方法在30min内完成了10种糖和糖醇的分离,灵敏度高,重现性好,可以用于无糖食品中糖和糖醇含量的测定。
译  名：
Simultaneous Determination by HPLC-ELSD of Ten Kinds of Sugars and Sugar Alcohols in Foods
作  者：
LIN Hui,YAN Chun-rong,XU Chun-xiang,CAI Jing,WANG Duo-jiao(Jiangsu Product Quality Testing and Inspection Institute,Nanjing 210007,China)
关键词：
high performance liquid chromatography(HPLC);evaporative light-scattering detector(ELSD);food;sugar;sugar alcohols
摘  要：
A high performance liquid chromatography with evaporative light-scattering detection(HPLC-ELSD) method was developed for the simultaneous determination of 5 sugars including fructose,glucose,sucrose,maltose,lactose and 5 sugar alcohols inlcuding erythritol,xylitol,sorbitol,mannitol,and maltitol in foods.Extracts of samples were separated on a Carbohydrate ES HPLC column at 30 ℃ using acetonitrile-water as mobile phase at a flow rate of 1.0 mL/min.The detector drift tube was kept at 95 ℃ and the nitrogen rate was set at 2.5 L/min.The results showed that the linear range of all these analytes was 0.2–20 mg/mL.The precision of the HPLC-ELSD method was < 5% in 6 replicate determinations,and average spike recoveries were 91.53%–105.2%.Successful separation and sensitive and reproducible detection of the sugars and sugar alcohols were achieved by this method within 30 min,thereby providing a promising approach for simultaneous determination of sugar and sugar alcohols in food.

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