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当前位置: 首页 > 文章 > HPLC法同时测定红曲色素中的红曲素和安卡红曲黄素 食品科学 2009,30 (8) 163-166
Position: Home > Articles > Simultaneous Detection of Monascine and Ankaflavine in Monascus Pigments by HPLC FOOD SCIENCE 2009,30 (8) 163-166

HPLC法同时测定红曲色素中的红曲素和安卡红曲黄素

作  者：
崔莉;胡晓丹;张德权
单  位：
中国农业科学院农产品加工研究所;北京林业大学生物科学与技术学院
关键词：
高效液相色谱法(HPLC);检测;红曲素;安卡红曲黄素
摘  要：
目的:建立同时测定红曲混合色素中的红曲素和安卡红曲黄素的高效液相色谱方法。方法:采用Shim-PackHRC-ODS色谱柱,甲醇-水溶液(80:20)为流动相,流速1.0ml/min,检测波长394nm,以自制的两种色素标准品定性定量。结果:该方法在10～500mg/L范围内呈良好的线性关系,回归方程和相关系数分别为:红曲素:Y=0.00015X+0.82365,R2=0.99988;安卡红曲黄素:Y=0.00031X-0.45,R2=0.99987。多次测定的相对标准偏差均小于4%,采用该方法对收集到的国内部分企业的红曲色素产品进行了检测。结论:该方法具有较好的重复性和稳定性,可用于红曲色素中红曲素和安卡红曲黄素的检测,进而可用于红曲色素产品组成和质量分析。
译  名：
Simultaneous Detection of Monascine and Ankaflavine in Monascus Pigments by HPLC
作  者：
CUI Li1,HU Xiao-dan2,ZHANG De-quan1,(1.Institute of Agro-Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193,China;2.College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing 100083,China)
关键词：
high performance liquid chromatography(HPLC);detection;monascine;ankaflavine
摘  要：
Objective:To establish an efficient method for simultaneously detecting both of monascine and ankaflavine in monascus pigments by high performance liquid chromatography(HPLC).Methods:With the self-made pigments as reference substances,monascine and ankaflavine were qualitatively and quantitatively analyzed adopting SHIM-PACK HRC-ODS C18 column and methanol-water(80:20,V/V) mixture as mobile phase under the condition of flow rate 1.0 ml/min and detection wavelength 394 nm.Results:The method has a good linear relationship in the range of 10 to 500 mg/L.The regression equation of monascine is Y = 0.00015x + 0.82365 with the determination coefficient of 0.99988,and the regression equation of ankaflavine is Y = 0.00031x-0.45 with the determination coefficient of 0.99987.Their relative standard deviations are both less than 4%.The detection results of monascine and ankaflavine contents in monascus pigment products collected from some companies in China by this method are satisfying.Conclusion:This method has a good reproducibility and stability,and can be used for detection of monascine and ankaflavine and analysis of compositions and quality of monascus pigment products.

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