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Position: Home > Articles > Effects of temperature and carbon source on growth and docosahexaenoic acid accumulation of Crypthecodinium cohnii Chinese Journal of Oil Crop Sciences 2013,35 (5) 597-603

温度和碳源对隐甲藻生长及DHA积累的影响

作  者:
郭小婧;龚阳敏;梁焯;万霞;胡传炯;刘姣;江木兰
单  位:
中国农业科学院油料作物研究所
关键词:
隐甲藻;培养条件;生物量;DHA
摘  要:
研究了隐甲藻(Crypthecodinium cohnii ATCC 30772)以摇瓶发酵培养方式获得最大生物产量和最高二十二碳六烯酸(docosahexaenoic acid,DHA)产量的培养条件。结果表明,有利于隐甲藻生长和DHA积累的最佳培养条件为:培养温度为22℃,先以12g/L葡萄糖为碳源培养4d,后再以3g/L醋酸钠为碳源培养4d。由此获得的生物产量为234.85mg/(L·d),DHA产量为17.45mg/(L·d),DHA含量为74.36mg/g菌体。
译  名:
Effects of temperature and carbon source on growth and docosahexaenoic acid accumulation of Crypthecodinium cohnii
作  者:
GUO Xiao-jing;GONG Yang-min;LIANG Zhuo;WAN Xia;HU Chuan-jiong;LIU Jiao;JIANG Mu-lan;Oil Crops Research Institute of Chinese Academy of Agriculture Sciences;Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture;
关键词:
Crypthecodinium cohnii;;Culture condition;;Biomass;;DHA
摘  要:
Culture conditions were adapted to obtain larger biomass and higher docosahexaenoic acid(DHA)yield of Crypthecodinium cohnii under flask fermentation level.Results showed that the optimal culture should be at 22℃,in carbon-source culture with 12g/L glucose for 4d and then shifted to the same media but with 3g/L sodium acetate instead for 4d.Under this optimal condition,the C.cohnii biomass yield,DHA yield and DHA content reached 234.85mg/(L·d),17.45mg/(L·d),74.36mg/g dry weight respectively.

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