当前位置: 首页 > 文章 > 氮限制对产油杜氏藻转录组的影响 中国油料作物学报 2016,38 (4) 524-528
Position: Home > Articles > Effect of nitrogen limitation on transcriptome of oleaginous Dunaliella parva Chinese Journal of Oil Crop Sciences 2016,38 (4) 524-528

氮限制对产油杜氏藻转录组的影响

作  者:
尚常花;王忠铭;袁振宏;谢君;万霞
单  位:
中国农业科学院油料作物研究所;中国科学院可再生能源重点实验室;华南农业大学新能源与新材料研究所
关键词:
杜氏藻;转录组;Illumina高通量测序;代谢途径
摘  要:
为通过改良产油杜氏藻制备生物柴油,利用Illumina Hiseq 2000平台对氮限制和氮源充足条件下的产油杜氏藻进行了转录组测序,筛选到1 529个差异表达基因。对这些差异表达基因进行分析,结果表明众多基因涉及内吞作用、甘油磷脂代谢、醚脂代谢,遗传物质的合成、传递和加工,次级代谢产物合成及氮代谢。
译  名:
Effect of nitrogen limitation on transcriptome of oleaginous Dunaliella parva
作  者:
SHANG Chang-hua;WANG Zhong-ming;YUAN Zhen-hong;XIE Jun;WAN Xia;Key Laboratory of Renewable Energy,Chinese Academy of Sciences,Guangdong Key Laboratory of New and Renewable Energy Research and Development,Guangzhou Institute of Energy Conversion,Chinese Academy of Sciences;Institute of New Energy and New Materials,South China Agricultural University;Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture;
关键词:
Dunaliella parva;;Transcriptome;;Illumina Hi-sequencing;;Metabolic pathway
摘  要:
To modifying oleaginous Dunaliella parva for biodiesel,transcriptome of Dunaliella parva was sequenced under nitrogen limitation and nitrogen sufficient condition by Illumina Hiseq 2000 platform. 1 529 differentially expressed genes were obtained. Resluts indicated that many differentially expressed genes were involved in endocytosis,glycerophospholipid metabolism,ether lipid metabolism,the biosynthesis,transport and process of genetic materials,biosynthesis of secondary metabolites and nitrogen metabolism.

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