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当前位置: 首页 > 文章 > 可可蔗糖磷酸合成酶基因家族进化及组织表达分析 热带作物学报 2015,36 (9) 1608-1613
Position: Home > Articles > Phylogeny and Expression Profile of the Sucrose Phosphate Synthase Gene Family in Cacao(Theobroma cacao L.) Chinese Journal of Tropical Crops 2015,36 (9) 1608-1613

可可蔗糖磷酸合成酶基因家族进化及组织表达分析

作  者：
李付鹏;秦晓威;伍宝朵;赵溪竹;王华;朱自慧;赖剑雄
单  位：
中国热带农业科学院香料饮料研究所
关键词：
可可;蔗糖磷酸合成酶;表达分析;系统进化
摘  要：
在高等植物中,蔗糖磷酸合成酶(Sucrose phosphate synthase,SPS)是蔗糖合成的限速酶。在多种植物中都发现了SPS基因,而可可中尚未见相关报道。通过分析可可基因组数据库,鉴定出4个SPS候选基因,依次命名为TcSPS1、TcSPS2、TcSPS3和TcSPS4。4个基因的编码区(CDS)长度在3 075~3 228 bp之间,外显子数目为12~14,预测蛋白的平均分子量为118.15 ku,等电点均小于7。进化分析结果表明SPS基因家族分成3个亚族;TcSPS1和TcSPS2属于ClassⅠ亚族,TcSPS3和TcSPS4分别属于ClassⅡ亚族和ClassⅢ亚族。实时荧光定量PCR分析结果表明,TcSPS1与TcSPS2在树皮和果实中高量表达,TcSPS3和TcSPS4主要在叶片中表达。伴随着叶片和花蕾生长发育,各TcSPS基因表达量均呈现出上升的趋势,表明其与主要光合产物--蔗糖的合成或再合成有密切联系,参与可可"源库"器官中光合产物分配。
译  名：
Phylogeny and Expression Profile of the Sucrose Phosphate Synthase Gene Family in Cacao(Theobroma cacao L.)
作  者：
LI Fupeng;QIN Xiaowei;WU Baoduo;ZHAO Xizhu;WANG Hua;ZHU Zihui;LAI Jianxiong;Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences;Ministry of Agriculture Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops;Tropical Spice and Beverage Germplasm Repository, Ministry of Agriculture;
关键词：
Theobroma cacao L.;;Sucrose phosphate synthase;;Expression profile;;Phylogenetic
摘  要：
In higher plants, sucrose phosphate synthase( SPS) is widely considered as a key gene for sucrose synthesis. Although several paralogous genes encoding different isozymes of SPS have been identified and characterized in multiple plant genomes, detailed information about the SPS genes is still lacking for cacao. The study reported that the identification of four novel SPS genes from economically important cacao tree were designated as Tc SPS1, Tc SPS2, Tc SPS3 and Tc SPS4. Sequences analyses revealed that CDS of Tc SPS1-4 were ranging from 3 075 bp to 3 228 bp, containing 12-14 exons. The average molecular weight of four predicted protein were 118.15 ku, and p Is of all proteins were less than 7. The expression patterns of the cacao SPS genes were investigated via Real-time PCR in various tissues, different developmental phases of leaf, flower bud. Tc SPS1 and Tc SPS2 were highly expressed in the bark and pods; Tc SPS3 and Tc SPS4 were predominantly expressed in the leaves. The transcription levels of Tc SPS genes were continuously increased along with the leaf and flower bud development. These results indicated that Tc SPS genes were significantly related with sucrose synthesis or re-synthesis in source and sink organs, and participate in photoassimilate translocation.

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