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Position: Home > Articles > Discrimination between Authentic and Fake Honey Based on β-Glucosidase Activity FOOD SCIENCE 2012,33 (16) 150-153

基于β-葡萄糖苷酶活力的蜂蜜真伪鉴别

作  者:
张金连;张翠平;郑火青;易松强;胡福良
单  位:
浙江大学动物科学学院
关键词:
蜂蜜;β-葡萄糖苷酶;真伪;鉴别
摘  要:
提出以β-葡萄糖苷酶活力为指标的蜂蜜真伪鉴别新方法,采用分光光度法分析不同来源蜂蜜中的β-葡萄糖苷酶活力,研究不同储存条件下蜂蜜中β-葡萄糖苷酶活力的变化规律,探讨掺入不同比例大米高果糖浆的蜂蜜中以β-葡萄糖苷酶活力的变化规律。结果表明:不同来源蜂蜜中均能检测出β-葡萄糖苷酶活力,而大米高果糖浆中检测不到该酶活力;不同浓度油菜蜜分别在-18、4、25(室温)、30℃条件下储存11个月,随着储存时间的延长,蜂蜜中β-葡萄糖苷酶活力变化较小;随着油菜蜜中大米高果糖浆的掺假比例增大,β-葡萄糖苷酶活力呈线性下降。因此,β-葡萄糖苷酶活力是检测蜂蜜是否掺假的一个有效定性指标。
译  名:
Discrimination between Authentic and Fake Honey Based on β-Glucosidase Activity
作  者:
ZHANG Jin-lian,ZHANG Cui-ping,ZHENG Huo-qing,YI Song-qiang,HU Fu-liang(College of Animal Sciences,Zhejiang University,Hangzhou 310058,China)
关键词:
honey;β-glucosidase;adulteration;identification
摘  要:
A new method using β-glucosidase activity was proposed to detect honey adulteration.The activities of β-glucosidase in honey from different plants,different storage conditions,and different proportions of high-fructose rice syrup were determined.The results showed that β-glucosidase activity was detectable in honey samples from different plants;however,no β-glucosidase activity was detected in honey adulterated with high-fructose rice syrup.β-glucosidase activity in rape honey showed little change during 11 months of storage at-18 ℃,4 ℃,room temperature or 30 ℃.With increasing high-fructose rice syrup adulteration,β-glucosidase activity revealed a linear decline.In conclusion,β-glucosidase activity can be considered as an effective indicator for the identification of honey adulteration.

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