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Position: Home > Articles > Detection of Cry2A Protein Based on Quartz Crystal Microbalance Technique FOOD SCIENCE 2014,35 (24) 209-212

Cry2A蛋白的石英晶体微天平检测

作  者:
杜方;黄新;纪淑娟
单  位:
沈阳农业大学食品学院;中国检验检疫科学研究院植物检疫研究所
关键词:
石英晶体微天平;Cry2A蛋白;转基因作物
摘  要:
目的:建立检测苏云金芽孢杆菌Cry2A蛋白的石英晶体微天平(quartz crystal microbalance,q CM)传感方法。方法:根据抗原抗体相互作用原理,利用q CM技术,在金片表面修饰抗原所对应的单克隆抗体,对苏云金芽孢杆菌Cry2A蛋白进行检测研究。结果:该方法灵敏度达到1 μg/m L,特异性好、重复性高。结论:该方法有利于为苏云金芽孢杆菌蛋白检测提供新思路,可以应用到实际样品的检测,在农产品转基因检测和进出口检验检疫中具有很好的应用前景。
译  名:
Detection of Cry2A Protein Based on Quartz Crystal Microbalance Technique
作  者:
DU Fang;HUANG Xin;JI Shu-juan;College of Food Science, Shenyang Agricultural University;Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine;
关键词:
quartz crystal microbalance (QCM);;Cry2A protein;;genetically modified crop (GMC)
摘  要:
Purpose: To establish a detection method for Cry2A protein by quartz crystal microbalance (QCM) sensing method. Methods: By using QCM, based on the principle of the interaction between antigen and antibody, the specific monoclonal antibody was modified on the gold surface for Cry2A protein detection. Results: The proposed method had a high sensitivity of 1 μg/m L, good specificity, and sufficient repeatability. Conclusion: This method may provide a new idea for the detection of Bacillus thuringiensis protein from genetically modified crops and thus has promising prospects for application in import and export inspection and quarantine supervision work.

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