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当前位置: 首页 > 文章 > 电刺激频率对肉鸡成肌细胞抗氧化能力及核因子E2相关因子2/抗氧化反应序列元件信号通路中抗氧化相关基因表达的影响 动物营养学报 2020 (11) 5415-5423
Position: Home > Articles > Effects of Electrical Stimulation Frequencies on Antioxidant Capacity and Expression of Antioxidant-Related Genes in Factor-Erythroid 2-Related Factor 2/Antioxidant Reaction Sequence Element Signaling Pathway in Broiler Myoblasts Chinese Journal of Animal Nutrition 2020 (11) 5415-5423

电刺激频率对肉鸡成肌细胞抗氧化能力及核因子E2相关因子2/抗氧化反应序列元件信号通路中抗氧化相关基因表达的影响

作  者：
胥蕾;张欣;黄华云;李宁;万晓莉;杨海明;王志跃
单  位：
扬州大学动物科学与技术学院;江苏省家禽科学研究所
关键词：
电刺激;肉鸡成肌细胞;抗氧化能力;Nrf2;NQO1
摘  要：
本试验旨在研究不同的电刺激频率对肉鸡成肌细胞抗氧化能力及核因子E2相关因子2(Nrf2)/抗氧化反应序列元件(ARE)信号通路中抗氧化相关基因表达的影响。试验将分化3 d的肉鸡成肌细胞分为对照组和5个电刺激组,每组3个重复。对照组不进行电刺激处理,处理组的电刺激频率分别为50、160、320、640和1 280 Hz,刺激电压和时间统一为30 V和10 s。电刺激处理后继续培养30 min,测定肉鸡成肌细胞抗氧化能力和Nrf2/ARE信号通路中抗氧化相关基因表达的变化。结果表明:不同的电刺激频率对细胞损伤程度无显著影响(P>0.05);320 Hz组细胞过氧化氢(H2O2)含量显著低于50、640和1 280 Hz组(P<0.05),还原型谷胱甘肽(GSH)含量和超氧化物歧化酶(SOD)活性显著高于对照组和640 Hz组(P<0.05);320 Hz组成肌细胞中Nrf2、Kelch样环氧氯丙烷相关蛋白-1(Keap1)和NAD(P)H醌氧还原酶1(NQO1)的mRNA相对表达量显著高于其他电刺激组(P<0.05),谷胱甘肽-S-转移酶T1(GSTT1)的mRNA相对表达量最高,与对照组和其他电刺激组均存在显著差异(P<0.05)。综上所述,在电刺激30 V和10 s的情况下,320 Hz的电刺激频率可以提高肉鸡成肌细胞的抗氧化能力和Nrf2/ARE信号通路中部分抗氧化相关基因的mRNA相对表达量,但并没有改变细胞形态或引起明显的细胞膜破损。
译  名：
Effects of Electrical Stimulation Frequencies on Antioxidant Capacity and Expression of Antioxidant-Related Genes in Factor-Erythroid 2-Related Factor 2/Antioxidant Reaction Sequence Element Signaling Pathway in Broiler Myoblasts
作  者：
XU Lei;ZHANG Xin;HUANG Huayun;LI Ning;WAN Xiaoli;YANG Haiming;WANG Zhiyue;College of Animal Science and Technology,Yangzhou University;Jiangsu Institute of Poultry Science;
关键词：
electrical stimulation;;broiler myoblasts;;antioxidant capacity;;Nrf2;;NQO1
摘  要：
This experiment was to investigate the effects of different electrical stimulation frequencies on the antioxidant capacity and expression of antioxidant-related genes in factor-erythroid 2-related factor 2(Nrf2)/antioxidant reaction sequence element(ARE)signaling pathway in broiler myoblasts. The myoblasts were divided into the control group and five electrical stimulation groups,with three replicates in each group. The myoblasts in control group were not treated with electrical stimulation. The myoblasts in the other groups were respectively stimulated with 50,160,320,640 and 1 280 Hz electricity combined with a stable voltage of 30 V and a stimulation period of 10 s. Cells were cultured for 30 min after the treatments. Then the changes of antioxidant capacity and expression of antioxidant-related genes in Nrf2/ARE signaling pathway were measured in broiler myoblasts. The results showed that different electrical frequencies had no significant effect on the degree of cell injury(P>0.05). The hydrogen peroxide(H2 O2)content in 320 Hz group was significantly lower than that in 50,640 and 1 280 Hz groups(P<0.05). The reduced glutathione(GSH)content and superoxide dismutase(SOD)activity in 320 Hz group were significantly higher than those in the control group and 640 Hz group(P < 0. 05). The mRNA relative expression levels of Nrf2,Kelch-like ECH-associated protein 1(Keap1)and NAD(P)H quinone oxidoreductase l(NQO1)were significantly higher in 320 Hz group than those in other electrical stimulation groups(P<0.05). The mRNA relative expression of glutathione-S-transferase T1(GSTT1)was the highest in 320 Hz group as compared with other groups(P<0.05). In conclusion,a stimulating frequency at 320 Hz can improve the antioxidant capacity and the expression of antioxidant-related genes in Nrf2/ARE signaling pathway without changing cell morphology or causing obvious damage to cell membrane in broiler myoblasts.[Chinese Journal of Animal Nutrition,2020,32(11):5415-5423]

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