摘 要：
利用配体交换反应将巯基修饰的"8-17"脱氧核酶("8-17"DNAzyme/"8-17"Dz)底物链(17S)通过Au-S共价键自组装在纳米金胶(AuNPs)表面,制得一种新型纳米复合物17S-AuNPs.利用紫外-可见吸收光谱和动态光散射(dynamic light scattering,DLS)对其进行了表征.紫外-可见吸收光谱表明17S-AuNPs在523 nm波长处有表面等离子共振峰,较AuNPs红移了3 nm;经DLS测定的17S-AuNPs的流体动力学平均直径为38.6 nm,较AuNPs的21.8 nm增大了16.8 nm.另外,利用紫外-可见光谱和目视比色法对AuNPs和17S-AuNPs的耐盐稳定性分别进行了比较研究,结果表明:随着水溶液中Na+浓度的逐渐提高,AuNPs在600 nm处的吸收峰不断增强,溶液颜色逐渐由红变成蓝紫色;而17S-AuNPs在600 nm处并未出现吸收峰,且溶液颜色一直保持为红色;表明17S-AuNPs纳米复合物的稳定性较AuNPs显著提高.
译 名：
Preparation,characterization and stability of "8-17" DNAzyme substrate-gold nanoparticle conjugates
单 位：
College of Food and Science Technology,Shanghai Ocean University,Shanghai 201306,China%Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation,Shanghai 201306,China
关键词：
DNAzyme%gold nanoparticle%self-assembly%stability
摘 要：
"8-17" DNAzyme substrate-gold nanoparticles conjugates(17S-AuNPs) were prepared by ligand exchanged reaction.It was characterized using dynamic light scattering(DLS) and UV-Vis absorption spectrum.The results indicated that the well-dispersed gold nanoparticles showed a surface plasma peak at 520 nm in the UV-Vis absorption spectrum,whereas the peak of 17S-AuNPs conjugates red shifted to wavelength 523 nm.Meanwhile,the hydrodynamic radius of AuNPs and 17S-AuNPs were confirmed to be 21.8 nm and 38.6 nm,respectively,using DLS.Moreover,the stability toward salt-induced aggregation of AuNPs and 17S-AuNPs was further investigated,respectively.As the concentrations of Na+ increased gradually,the absorptiom peak of AuNPs increased at 600 nm,following with a color change from red to blue.For 17S-AuNPs a peak at 600 nm is not observed,and the color of the 17S-AuNPs solution keeps red.The aforementioned results indicated that 17S-AuNPs showed a higher stability than that of AuNPs.
