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Position: Home > Articles > Optimization of the Conditions for Soybean Glycinin Extraction and Analysis of 7S and 11S in Soybean Protein Acta Agriculturae Universitatis Jiangxiensis 2014 (5) 1139-1144

大豆球蛋白提取条件优化及7S和11S亚基的鉴定

作  者:
彭棋;阮记明;黄冬艳;王自蕊;黄柳梅;熊平文;黄建珍
单  位:
江西农业大学动物科学技术学院
关键词:
大豆球蛋白;提取;7S伴大豆球蛋白;11S球蛋白;SDS-PAGE
摘  要:
大豆球蛋白是豆粕饲料的主要营养成分之一,对豆粕饲料的营养品质有着重要影响,其中7S伴大豆球蛋白及11S球蛋白是大豆球蛋白最重要的成分。研究分析了在不同提取液,不同的pH,不同的温度,不同浓度的Tris-HCl提取液及不同料液比对豆粕大豆球蛋白提取的影响,并且对7S伴大豆球蛋白和11S球蛋白进行了分析。最终SDS-PAGE结果显示55℃、0.05 mol/L、pH9.0的Tris-HCl溶液在料液比为1∶10时能够提取较多的球蛋白,并且7S伴大豆球蛋白由3个亚基组成,其相对分子量分别为60 466 Da,55 029 Da和49 043 Da,11S球蛋白也由3个亚基组成,其相对分子量分别为44 634 Da,39 779 Da和17 952 Da。
译  名:
Optimization of the Conditions for Soybean Glycinin Extraction and Analysis of 7S and 11S in Soybean Protein
作  者:
PENG Qi;RUAN Ji-ming;HUANG Dong-yan;WANG Zi-rui;HUANG Liu-mei;XIONG Ping-wen;HUANG Jian-zhen;College of Animal Science and Technology,Jiangxi Agricultural University;
关键词:
soybean glycinin;;extraction;;7s-conglycinin globulins;;11S globulins;;SDS-PAGE
摘  要:
Soybean glycinin,which is one of the major compositions in soybean meal,greatly influences the quality of soybean meal,and the most important components of glycinin are 7s- conglycinin globulins and 11 S globulins. The objective of the study was to decide the optimal conditions for extraction of soybean protein and to analyze 7s- conglycinin globulins and 11 S globulins. The results of SDS- PAGE showed that 0. 05 M Tris-HCl(pH9. 0,55 ℃) was the optimal condition for the extraction of soybean glycinin with the sample- solution ratio 1 ∶ 10,and 7s- conglycinin globulins were composed of three subunits with molecular weight of60466 Da,55029Da and 49043 Da,respectively and 11 S globulins were also composed of three subunits with molecular weight of 44634 Da,39779Da and 17952 Da,respectively.

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