译 名：
The Aristolochic Acid A's Abstraction in Manchurian Dutchmanspipe Stem and Contet Assaying
作 者：
WANG Dian-ren,WANG Yan-ying,LU Yan,SHEN Hong,ZHANG Zhong-wen,WU Guo-juan(Beijing University of Agricultural Animal Science and Technical Department,Beijing 102206,China)
关键词：
manchurian dutchmanspipe stem;aristolochic acid A;extraction;assaying
摘 要：
Extracted aristolochic con-acid by dehydrate alcohol as the solvent from 150 g manchurian dutchmanspipe stem powder,get crude extract after rotary evaporation and vacuum dehydration,and dissolved extractum with NaHCO3 saturated solution,adjusted the pH to 3 after delamination.Used silica gel G chromatographic colum to laminar analysis.The eluent was made up of chloroform∶acetidin∶methylbenzol(2∶1∶1).Then get flav-crystal 0.389g after recrystallization,identified and assayed the content with R-HPLC.the result showed that the flav-crystal was aristolochic acid A monomer,and the content reached 99.2%.The methold of extraction could get high purity aristolochic acid A,and it was a high performance way.
