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当前位置: 首页 > 文章 > 53株禽致病性大肠杆菌的耐药表型及耐药基因的检测 畜牧与兽医 2017,49 (5) 134-141
Position: Home > Articles > Detection of resistance phenotypes and resistance determinants in 53 avian pathogenic Escherichia coli strains Animal Husbandry & Veterinary Medicine 2017,49 (5) 134-141

53株禽致病性大肠杆菌的耐药表型及耐药基因的检测

作  者：
于静晨;王虹;李鑫;李亚芯;汤芳;戴建君
单  位：
南京农业大学动物医学院
关键词：
禽致病性大肠杆菌;耐药性表型;耐药基因
摘  要：
为了解禽致病性大肠杆菌耐药表型及耐药基因的情况,选取江苏、安徽等地分离的53株禽致病性大肠杆菌,采用药敏纸片法对9种抗菌药物进行药敏试验,并对四环素类tet(A)、tet(B)、tet(C)、tet(W)、tet(M)、tet(O)、tet(K)、tet(L)耐药基因,喹诺酮类GryA、ParC耐药基因,磺胺类sulⅠ、sulⅡ、sulⅢ耐药基因,β-内酰胺类SHV、CTX-M、ompCA耐药基因,氨基糖苷类aac(3)-Ⅰ、aac(3)-Ⅱ、aac(6')-Ⅰb、aac(6')-Ⅱ、ant(3″)-Ⅰ、armA、rmtA、rmtB、rmtC、rmtD、npmA耐药基因进行PCR检测。结果显示:53株禽致病性大肠杆菌对磺胺异噁唑、环丙沙星、氨苄西林、多西环素的耐药率较高,分别为88.68%(47/53)、71.71%(38/53)、86.79%(46/53)、75.47%(40/53)。其中50株禽致病性大肠杆菌表现为多重耐药,耐4、5、6种药物的现象最为普遍,且不同地区菌株存在差异。tet(A)是四环素耐药基因中最为流行的一种耐药基因(52.83%,28/53),喹诺酮类耐药基因主要由gryA(94.33%,50/53)、parC(94.33%,50/53)基因编码,耐磺胺类药物sulⅠ、sulⅡ、sulⅢ基因均有检出,分别为96.23%(52/53)、98.11%(48/53)、86.79%(46/53),耐β-内酰胺类药物中仅检出ompC A基因(30.19%,16/53),在检测的11种耐氨基糖苷类耐药基因中,最为流行为aac(3)-Ⅱ、aac(6')-Ⅰb、ant(3″)-Ⅰ基因,分别为92.59%(49/53)、98.11%(52/53)、100%(53/53)。耐药基因与相关耐药菌株检出率基本呈正相关。试验结果表明:53株禽致病性大肠杆菌耐药性高,耐药谱广,耐药基因流行现象十分普遍。本试验结果能为禽致病性大肠杆菌的耐药现状与临床用药提供理论指导。
译  名：
Detection of resistance phenotypes and resistance determinants in 53 avian pathogenic Escherichia coli strains
作  者：
YU Jingchen;WANG Hong;LI Xin;LI Yaxin;TANG Fang;DAI Jianjun;College of Veterinary Medicine,Nanjing Agricultural University;
关键词：
avian pathogenic Escherichia coli(APEC);;antibiotic resistance phenotype;;resistance determinants
摘  要：
To investigate the antibiotic resistance and resistance genes in avian pathogenic Escherichia coli(APEC)isolated from Jiangsu and Anhui provinces,the susceptibility to 9 antibiotics were conducted on 53 isolates of APEC.Tetracycline-resistant genes(tet(A),tet(B),tet(C),tet(W),tet(M),tet(O),tet(K),tet(L)),quinolone-resistant genes(gryA,parC),sulfisoxazole-resistant genes(sulⅠ,sulⅡ,sulⅢ),β-lactamase-resistant genes(SHV,CTX-M,ompC A)and aminoglycoside-resistant genes(aac(3)-Ⅰ,aac(3)-Ⅱ,aac(6')-Ib,aac(6')-Ⅱ,ant(3")-Ⅰ,armA,rmtA,rmtB,rmtC,rmtD,npmA)were detected by using conventional PCR.The results showed that the resistance rates of the 53 isolates to sulfisoxazole,ciprofloxacin,ampicillin and deoxytetracycline were high,which were 88.68%(3/53),71.71%(38/53),86.79%(46/53)and 75.47%(40/53),respectively.Among them,50 isolates were multidrug resistant strains,and the number of APEC isolates,which were resistant to 4,5 or 6 antibiotics was relatively larger than others.Also,the resistant rates varied in different areas.As revealed by detecting resistance determinants,tet(A)was the most prevalent gene among tetracycline,which accounted for 52.83%(28/53).GryA(94.33%,50/53)and parC(94.33%,50/53)were main quinolone-resistant genes in APEC.Three kinds of sulfisoxazole-resistant genes,sulⅠ(96.23%,48/53),sulⅡ(98.11%,52/53)and sulⅢ(86.79%,46/53)were all detected in APEC,while ompC A(30.19%,16/53)was the mainβ-lactamase resistance gene.Among 11 aminoglycoside resistance genes,aac(3)-Ⅱ(92.59%,49/53),aac(6')-Ⅰ(98.11%,52/53)and ant(3")-Ⅰ(100%,53/53)were most common resistance genes in APEC.The detection rate of resistance phenotype was positively correlative to the relevant resistance genes.The result indicated that the resistance rate of APEC was high with wide drug resistant spectrum,and the prevalence of drug resistant genes was very common.The studycan provide some references for clinical research andrational drug usage.

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