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当前位置: 首页 > 文章 > 壳寡糖诱导烟草防御酶系活性变化及PR-1a基因表达研究 植物病理学报 2010,40 (1) 99-102
Position: Home > Articles > Changes of defensive enzymes and PR-1a gene expression of tobacco induced by chito-oligosaccharides Acta Phytopathologica Sinica 2010,40 (1) 99-102

壳寡糖诱导烟草防御酶系活性变化及PR-1a基因表达研究

作  者：
商文静;吴云锋;赵小明;杜昱光;商鸿生
单  位：
西北农林科技大学植物保护学院陕西省农业分子生物学重点实验室;中国科学院大连化学物理研究所
摘  要：
The activity change of defensive enzymes and PR-1a gene expression of tobacco (Nicotiana tabacum) seedling induced by chito-oligosaccharides were studied. The results showed that high level systemic acquired resistance (SAR) was expressed in tobacco plants treated with chito-oligosaccharides solution at the concentration of 50 μg/mL. PAL activity increased greatly with 2 peaks, the activity of SOD decreased initially followed by an increase with higher increment, and the activity of POD peaked early followed by a gentle fall in chito-oligosaccharide treated plants. The PR-1a gene was strongly expressed in tobacco due to systemic acquired resistance induced by chito-oligosaccharides. At 168 h after inoculation the expression quantity (co-pies/2 μL) of PR-1a gene was increased to 2 469.6 in treated tobacco leaf, reached 392.6% than that at 0 h after inoculation,it was increased 3.05 times of that in untreated control.
译  名：
Changes of defensive enzymes and PR-1a gene expression of tobacco induced by chito-oligosaccharides
作  者：
SHANG Wen-jing1, WU Yun-feng1, ZHAO Xiao-ming2, DU Yu-guang2, SHANG Hong-sheng1 (1College of Plant Protection, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100,China; 2Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China)
关键词：
chito-oligosaccharides; SAR; defensive enzyme; PR-1a gene
摘  要：
The activity change of defensive enzymes and PR-1a gene expression of tobacco (Nicotiana tabacum) seedling induced by chito-oligosaccharides were studied. The results showed that high level systemic acquired resistance (SAR) was expressed in tobacco plants treated with chito-oligosaccharides solution at the concentration of 50 μg/mL. PAL activity increased greatly with 2 peaks, the activity of SOD decreased initially followed by an increase with higher increment, and the activity of POD peaked early followed by a gentle fall in chito-oligosaccharide treated plants. The PR-1a gene was strongly expressed in tobacco due to systemic acquired resistance induced by chito-oligosaccharides. At 168 h after inoculation the expression quantity (co-pies/2 μL) of PR-1a gene was increased to 2 469.6 in treated tobacco leaf, reached 392.6% than that at 0 h after inoculation,it was increased 3.05 times of that in untreated control.

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