中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 酶联免疫吸附分析法测定土壤中的毒死蜱 河南农业科学 2005,34 (8) 54-57
Position: Home > Articles > Determination of Chlorpyrifos in Soil by ELISA Journal of Henan Agricultural Sciences 2005,34 (8) 54-57

酶联免疫吸附分析法测定土壤中的毒死蜱

作  者：
韩丽君;钱传范;李文明;江树人;王小如
单  位：
中国农业大学理学院;河南农业大学植物保护学院
关键词：
酶联免疫吸附测定法;毒死蜱;土壤;残留检测
摘  要：
采用自制多克隆抗体,考察了pH值和甲醇含量对竞争反应的影响,并对反应体系进行了优化,最终建立了土壤中毒死蜱的残留检测ELISA方法。试验表明,毒死蜱对抗体竞争性结合的I50为76.8μg/L,方法检出限为5.2μg/L。土壤样品经简单前处理后,分别采用ELISA和GC测定,用ELISA方法测得土壤中毒死蜱的添加回收率为80.33%~83.36%,CV为5.28%~9.83%,最低检出限为0.005mg/kg,与GC测定结果基本相当,符合农药残留分析的要求。
译  名：
Determination of Chlorpyrifos in Soil by ELISA
作  者：
HAN Li-jun,QIAN Chuan-fan,LI Wen-ming,JIANG Shu-ren,WANG Xiao-ru(1.College of Science, China Agricultural University, Beijing 100094, China;2.College of Plant Protection,Henan Agricultural University,Zhengzhou 450002,China)
关键词：
ELISA; Chlorpyrifos; Soil; Pesticides residue test
摘  要：
Using the self-made poly-clonal antibody, the affect of pH and the methanol content on the competitive reaction were investigated, and the reaction system was optimized. An indirect enzyme-linked immunosorbent assay (ELISA) for the determination of chlorpyrifos residue in soil was developed. The results showed that the chlorpyrifos concentration(I_(50))that inhibits 50% of antibody-antigen binding was 76.8 μg/L,the detection limit I_(20) was 5.2 μg/L. Soil samples were detected with ELISA and GC after simple pre-procession. The fortified recovery of ELISA were 80.33%-(83.36%,)CV were 5.28%-9.83%, the determination limit was 0.005 mg/kg. The results of the ELISA were verified by GC method.

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46