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当前位置: 首页 > 文章 > 酶法提取金针菇菇根寡糖的研究初报 山东农业科学 2015 (2) 114-118
Position: Home > Articles > Preliminary Study on Enzymatic Extraction of Enoki Mushroom Root Oligosaccharides Shandong Agricultural Sciences 2015 (2) 114-118

酶法提取金针菇菇根寡糖的研究初报

作  者：
吕慧娟;郭兵;韩建东;宫志远
单  位：
青岛农业大学生命科学学院/山东省应用真菌省级重点实验室;山东省农业科学院农业资源与环境研究所
关键词：
金针菇菇根;多糖;酶解;寡糖
摘  要：
分别采用木瓜蛋白酶和溶壁酶降解金针菇菇根水提后的多糖,以提取还原性寡糖,并通过单因素试验和正交试验确定适合两种酶的最佳提取工艺。结果表明,木瓜蛋白酶降解菇根多糖的最佳工艺条件为酶解0.5 h、酶底比为4.5%、p H值6.0、温度为35℃;溶壁酶的最佳工艺条件为酶解1.5 h、酶底比为2.0%、p H值5.0、温度为50℃。采用不同的酶降解金针菇多糖,其还原性寡糖的得率不同,其中,溶壁酶降解所得还原性寡糖得率为6.8768%,木瓜蛋白酶降解所得还原性寡糖得率为6.1368%。
译  名：
Preliminary Study on Enzymatic Extraction of Enoki Mushroom Root Oligosaccharides
作  者：
Lü Huijuan;Guo Bing;Han Jiandong;Gong Zhiyuan;College of Life Science,Qingdao Agricultural University/Shandong Provincial Key Lab of Agricultural Applied Mycology;Institute of Agricultural Resources and Environment,Shandong Academy of Agricultural Sciences;
关键词：
Enoki mushroom root;;Polysaccharides;;Enzymolysis;;Oligosaccharide
摘  要：
In order to obtain oligosaccharides,the papain and lywallzyme were used respectively to degrade the polysaccharides extracted from enoki mushroom root with water. The optimum enzymolysis conditions for two enzymes were optimized through single- factor experiment and orthogonal test. The results showed that the optimum conditions for papain were enzymolysis for 0. 5 hours,the ratio of enzyme to substrate as 4. 5%,p H 6. 0 and the temperature as 35℃. The optimum conditions for lywallzyme were enzymolysis for 1. 5 hours,the ratio of enzyme to substrate as 2. 0%,p H 5. 0 and the temperature as 50℃. Using different enzymes to degrade enoki mushroom polysaccharides,the production of oligosaccharides was different. The yield of reducing oligosaccharide obtained by lywallzyme and papain were 6. 8768% and 6. 1368% respectively.

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