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Position: Home > Articles > Research on Diversity of Bacullus Species in Peanut Continuous Cropping Red Soils and Bio-preparation Effect of Bacullus Soils 2012,44 (5) 776-781

花生连作红壤芽孢杆菌的群落多样性及其生防效果研究

作  者:
李婧;陈广波;张坤;崔中利;曹慧
单  位:
南京农业大学农业部农业环境微生物工程重点开放实验室
关键词:
花生连作;微生物多样性;芽孢杆菌;RFLP分析;枯草芽孢杆菌
摘  要:
芽孢杆菌是土壤中常见的微生物类群,在土壤生态系统中占据重要地位。本研究发现低丘红壤区花生连作红壤中芽孢杆菌的数量为2.0×105~2.3×105cfu/g干土。通过构建3个样品的基因文库,共得到9个种类;16S rDNA测序结果表明该地区芽孢杆菌的优势类型有短小芽孢杆菌、枯草芽孢杆菌、高地芽孢杆菌,分别占总克隆的54%、12%和14%。并研究了施用不同微生物制剂对连作花生产量和生育性状的影响,结果显示,施用光合细菌和枯草芽孢杆菌能够在一定程度上提高花生的产量,枯草芽孢杆菌和光合细菌混合施用的增产效果最好,比对照提高了32.14%。
译  名:
Research on Diversity of Bacullus Species in Peanut Continuous Cropping Red Soils and Bio-preparation Effect of Bacullus
作  者:
LI Jing,CHEN Guang-bo,ZHANG Kun,CUI Zhong-li,CAO Hui(Key Laboratory of Microbiological Engineering of Agricultural Environment,MOA,Nanjing Agricultural University,Nanjing 210095,China)
关键词:
Peanut continuous cropping,Microbial diversity,Bacuillus spp.,RFLP analysis,Bacullus subtilis
摘  要:
Bacullus spp.are the common microorganisms in soils,occupying an important position in soil ecosystem.Our study found that the total number of culturable Bacullus spp.was 2.0×105-2.3×105cfu/g dry soil in peanut continuous cropping red soils.9 OUTs restriction endonuclease types were obtained in the three gene libraries.B.pumilus,B.subtilis and B.altitudinis,as the three main restriction types,accounted for 54%,12% and 14% respectively of the total 16S rDNA clones.The application of photosynthetic bacteria and bacillus subtilis could increase peanut yield,particularly when they mixed which could increased the yield by 32.14% compared to the control.

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