当前位置: 首页 > 文章 > 禽6型副粘病毒V蛋白对抗宿主细胞干扰素β的产生 安徽农业科学 2015 (1) 130-131
Position: Home > Articles > The V Protein of Avian Paramyxovirus Type 6 Antagonizes Beta Interferon Production of Host Cells Journal of Anhui Agricultural Sciences 2015 (1) 130-131

禽6型副粘病毒V蛋白对抗宿主细胞干扰素β的产生

作  者:
田志革;柴洪亮;华育平
单  位:
东北林业大学野生动物资源学院
关键词:
副粘病毒;V蛋白;对抗;干扰素β
摘  要:
[目的]研究禽6型副粘病毒V蛋白对抗宿主细胞干扰素β的产生的机制。[方法]构建真核表达禽6型副粘病毒P、V、W蛋白的重组载体,分别与IFN-β-Luc、NF-κB-Luc、PRDⅢ/I-Luc、AP-1-luc报告质粒共转染HEK-293T细胞,接种仙台病毒刺激细胞后,测定细胞内萤火虫荧光素酶及海肾荧光素酶活性。[结果]禽6型副粘病毒V蛋白能通过降低NF-κB的活性,进而显著抑制宿主细胞产生IFN-β。[结论]该研究初步阐明了禽6型副粘病毒V蛋白对抗宿主天然免疫的机制。
译  名:
The V Protein of Avian Paramyxovirus Type 6 Antagonizes Beta Interferon Production of Host Cells
作  者:
TIAN Zhi-ge;CHAI Hong-liang;HUA Yu-ping;Department of Wildlife Resource,Northeast Forestry University;
关键词:
Prarmyxovirus;;V protein;;Antagonize;;Beta interferon
摘  要:
[Objective]To study the mechanism of avian paramyxovirus type 6 V protein antagonizing beta interferon production. [Method]The study constructed the combine plasmids p CDNA-P,p CDNA-V,p CDNA-W for expressing protein P,V,W,respectively. The 293 T cells were cotransfected with combine plasmids and report gene plasmids,after Se V stimulating,the firely luciferase and Renilla luciferase activities were determined. [Result] The V protein remarkably inhibited beta interferon production by decreasing the NF-κB activity. [Conclusion] The study clarified the mechanism of V protein antagonizing innate immunity of host cells preliminarily.

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