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Position: Home > Articles > Preparing Androstenones by Microbial Degradation of Phytosterol in Biphasic System Transactions of the Chinese Society for Agricultural Machinery 2008,39 (9) 85-88+92

双液相系统中生物降解植物甾醇制备雄烯二酮工艺

作  者:
杨英;姜绍通;操丽丽;李悦
单  位:
合肥工业大学生物与食品工程学院
关键词:
雄烯二酮;双相系统;植物甾醇;生物降解
摘  要:
在双液相系统中,研究植物甾醇生物降解制备雄烯二酮(AD)的工艺,并考察底物投料量以及添加表面活性剂、羟化酶抑制剂对降解的影响。结果表明:葵花油是分枝杆菌降解甾醇生成AD的最佳有机溶剂,底物投料量以及羟化酶抑制剂对转化有一定的影响。通过正交试验,确定了葵花油/水系统最优转化条件:油相浓度为20%,底物投料量为0.4%,添加剂为0.005%氯化汞,此工艺条件下,AD转化率高达83.4%。
译  名:
Preparing Androstenones by Microbial Degradation of Phytosterol in Biphasic System
作  者:
Yang Ying Jiang Shaotong Cao Lili Li Yue(Hefei University of Technology,Hefei 230009,China)
关键词:
Androst-4-ene-3,17-dione,Biphasic systems,Phytosterol,Microbial degradation
摘  要:
The technology of obtaining androstenones(AD) from phytosterol by microbial degradation in biphasic systems and the effects of substrate concentration,surfactants and 9α-hydroxylase inhibitor on biotransformation were studied by experiment.The results show that sunflower oil-aqueous biphasic system is the most suitable for bioconversion,and the concentration of substrate and low-concentration of 9α-hydroxylase inhibitor can increase the rate of biotransformation to a certain extent.Finally,the sunflower oil-aqueous biphasic system of fermentation is optimized by orthogonal experiment,and the optimal condition is as following: systems with the concentration of 20% sunflower oil,0.4% substrate and 0.005% HgCl2.Under this condition the transformation rate of AD is 83.4%.

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