单 位：
南京农业大学动物医学院;中国农业科学院上海兽医研究所
摘 要：
采用两种不同的色谱条件测定吡喹酮注射剂中吡喹酮的含量,色谱柱为迪马D iamonsil C18(4.6 mm×250 mm,5μm)柱,流动相为甲醇-水及乙腈-水,检测波长为263 nm及210 nm,流速1mL/m in。结果显示,在两个不同的检测范围内,线性关系均良好(分别为r=0.999 8和r=0.999 6),吡喹酮的平均回收率为100.38%和99.90%,RSD=1.13%和1.1%(n=9)。两种方法简便易行,精密度、回收率、重现性均符合要求。结果表明,两种方法均可用于吡喹酮注射剂的含量测定。
译 名：
Comparition on Detemination of the Content of Praziquantel Injection
作 者：
LIU Fen1,2,FEI Chenzhong2,XUE Fei-qun2,JIANG Shan-xiang1(1.College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095;2.Shanghai Veterinary Research Institute,The Chinese Academy of Agricultural Sciences,The Key Open Laboratory for Animal Parasitology,Ministry of Agricultural/Key OpenLaboratory of Veterinary Drug Safety Evaluation and Residues of Chinese Academy of Agricultural Sciences,Shanghai 200241;China)
关键词：
PZQ injection;HPLC;determination of the content
摘 要：
To establish two HPLC method for determining the content of PZQ in the injection.The DIKMA Diamonsil C18(4.6 mm×250 mm,5 μm) Column was adopted.The mobile phase was V(methanol)∶V(water)and V(acetonitrile)∶V(water),the detective wavelength was 263 nm and 210 nm,the flow rate was 1 mL/min.The two methods were both linear within r=0.999 8/0.999 6.The mean recovery was 100.38% and 99.90%,RSD=1.13% and 1.1%(n=9).They turned out to be convienient method with good precision.These results showed that two methods both could be used to determine the content of PZQ in the injection.
