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Position: Home > Articles > Determination of monosaccharides and oligosaccharides in figwort root by ion chromatography Science and Technology of Food Industry 2012,33 (7) 340-343

离子色谱法测定玄参中的单糖和低聚寡糖

作  者：
熊建飞;周光明;何强;许丽;张磊;吴利敏
单  位：
西南大学化学化工学院;重庆大学三峡库区生态环境教育部重点实验室
关键词：
阴离子交换;脉冲安培;等度洗脱;超声水提;玄参;单糖;低聚寡糖
摘  要：
建立阴离子交换色谱-脉冲安培检测法测定玄参中的葡萄糖、蔗糖、果糖和水苏糖的方法。以METROSEPCARB1(150mm×4.0mm)阴离子交换柱为分离柱,脉冲安培检测,30mmol/LNaOH溶液为淋洗液等度洗脱,超声水提法提取玄参中的糖。峰面积的相对标准偏差为0.56%～9.01%,线性范围1～50mg/L,检出限为0.0452～0.0921mg/L,相关系数R为0.9931～0.9998,加标回收率在96.52%～109.56%之间。该方法预处理简单,准确度高,适用于快速测定玄参中的单糖和低聚寡糖。
译  名：
Determination of monosaccharides and oligosaccharides in figwort root by ion chromatography
作  者：
XIONG Jian-fei1,ZHOU Guang-ming1,HE Qiang2,,XU Li1,ZHANG Lei1,WU Li-min1(1.Key Laboratory on Luminescence and Real-Time Analysis(Southwest University),Ministry of Education,School of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China;2.Key Laboratory of Three Gorges Reservoir Region's Eco-Environment,Ministry of Education,Chongqing University,Chongqing 400045,China)
关键词：
anion exchange chromatography;pulsed amperometric detection;isocratic elution;ultrasonoscope;figwort root;monosaccharides;oligosaccharides
摘  要：
An anion exchange chromatography-pulsed amperometric detection method has been proposed for determination of glucose,sucrose,fructose,stachyose in figwort root.The analytical column was METROSEP CARB 1(150mm×4.0mm).The elution was performed using 30mmol/L sodium hydroxide isocratic elution and the column temperature was 32℃.A ultrasonoscope was used for extracting sugars.The RSD of area ranged from 0.56% to 9.01%,the linear range was 1~50mg/L,the detection limits was 0.0452~0.0921mg/L,the related coefficient(R)was 0.9931~0.9998,and the spike recoveries ranged from 96.52% to 109.56%.The method was simple and had a high sensitivity.So this method can be applied for the determination of monosaccharides and oligosaccharides in figwort root.

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