中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 分段盐析联合两步柱层析纯化巢湖蓝藻藻蓝蛋白 安徽农业大学学报 2018 (3) 487-491
Position: Home > Articles > Purification of phycocyanin from Chaohu cyanobacteria by fractional salting out and two steps column chromatography Journal of Anhui Agricultural University 2018 (3) 487-491

分段盐析联合两步柱层析纯化巢湖蓝藻藻蓝蛋白

作  者：
苏雨;张发宇;余金卫;汪家权;许子牧
关键词：
藻蓝蛋白;两步盐析;阴离子交换;羟基磷灰石
摘  要：
以巢湖打捞的新鲜蓝藻藻泥为处理对象,利用冻融破壁的方式获得藻蓝蛋白(phycocyanin,PC)粗提液。采取两步盐析初步纯化粗提液获得PC,再用纤维素(Cellufine A-500)阴离子交换树脂中度纯化PC,最后用羟基磷灰石(HA)柱高度纯化PC。结果表明,在室温25℃下,两步盐析(NH_4)_2SO_4的最佳摩尔浓度分别为1.0mol·L~(-1)和1.8 mol·L~(-1),PC纯度(A_(620)/A_(280))达到2.168,PC回收率可达37.5%。再经Cellufine A-500和HA两步柱层析纯化,PC纯度(A_(620)/A_(280))分别达到3.211和4.133,回收率分别为20.3%和7.2%,纯度达到试剂级纯度要求。
译  名：
Purification of phycocyanin from Chaohu cyanobacteria by fractional salting out and two steps column chromatography
作  者：
SU Yu;ZHANG Fayu;YU Jinwei;WANG Jiaquan;XU Zimu;School of Resources and Environmental Engineering, Hefei University of Technology;Hefei Dongfangmeijie Molecular Materials Technology Co.Ltd.;
单  位：
SU Yu%ZHANG Fayu%YU Jinwei%WANG Jiaquan%XU Zimu%School of Resources and Environmental Engineering, Hefei University of Technology%Hefei Dongfangmeijie Molecular Materials Technology Co.Ltd.
关键词：
phycocyanin;;two-step salt precipitation;;anion exchange;;hydroxyapatite
摘  要：
The fresh algae mud from Chaohu was used to obtain the crude extract of phycocyanin(PC) using freezing and thawing. The crude extract was purified by two-step salting-out, and the PC was purified by cellulose(Cellufine A-500) anion exchange resin and finally purified by hydroxyapatite(HA) column. The results showed that At room temperature(25℃), the optimum molar concentrations of two-step salting-out(NH_4)_2SO_4 were 1.0 mol·L~(-1) and 1.8 mol·L~(-1), respectively. The PC purity(A_(620)/A_(280)) reached to 2.168 and the PC recovery was 37.5 %. The purity of PC(A_(620)/A_(280)) reached 3.211 and 4.133, respectively, and the recovery was 20.3% and 7.2%, respectively, which reaches the reagent grade purity.

相似文章

导出引用
计量
文章访问数: 21
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46