当前位置: 首页 > 文章 > 牛奶中植物成分的PCR检测方法研究 食品科学 2008,29 (6) 234-236
Position: Home > Articles > Detection of Plant Ingredient in Milk by PCR Method FOOD SCIENCE 2008,29 (6) 234-236

牛奶中植物成分的PCR检测方法研究

作  者:
覃芳芳;邓鸿铃;郭新东;罗海英;吴玉銮
单  位:
广州市产品质量监督检验所
关键词:
DNA;PCR;牛奶;植物成分;掺入
摘  要:
建立了应用PCR技术鉴别牛奶饮料中植物成分的方法。将酚仿抽提DNA的方法与前处理相结合,得到了一种快速简便的从牛奶中提取DNA的方法,整个DNA提取的过程时间为2h左右。分析发现提取的牛奶饮料DNA完全可以进行PCR检测,并且该方法可检测到牛奶中掺入的植物成分低至0.1%。
译  名:
Detection of Plant Ingredient in Milk by PCR Method
作  者:
QIN Fang-fang,DENG Hong-ling,GUO Xin-dong,LUO Hai-ying,WU Yu-luan (Guangzhou Product Quality Supervision and Testing Institute, National Centre for Quality Supervision and Testing of Processed Food (Guangzhou), Guangzhou 510110, China)
关键词:
DNA;PCR;milk;plant element;adulteration
摘  要:
The PCR technology was used in the detection of plant ingredient in milk drink. Combined with pre-operation and phenol-chloroform extraction, a rapid and simple method of DNA extraction from milk drinks was found, and DNA extraction took only two hours. The quality of DNA is sufficient for PCR analysis. Moreover, 0.1% plant ingredient in milk drink can be detected by this method.

相似文章

计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊