当前位置: 首页 > 文章 > 小麦幼苗根系在Cr~(6+)胁迫下生长和DNA含量的变化 安徽农业科学 2011,39 (14) 83-84+123
Position: Home > Articles > Research on the Root Grown of Wheat Seedling under Stress of Cr~(6+) and the Variation in Content of DNA Journal of Anhui Agricultural Sciences 2011,39 (14) 83-84+123

小麦幼苗根系在Cr~(6+)胁迫下生长和DNA含量的变化

作  者:
黑淑梅
单  位:
延安大学生命科学学院
关键词:
Cr6+;小麦幼苗;根系;DNA含量
摘  要:
[目的]研究重金属Cr6+对小麦幼苗根系生长和DNA含量的影响。[方法]用浓度5~100 mg/L Cr6+分别处理3和10 d龄小麦幼苗,测定生长指标,采用紫外分光光度法和电泳法测定DNA含量变化。[结果]除了Cr6+>20 mg/L时,3d龄幼苗的根数小于对照根数外,其余处理根数均增加;而根长、根系鲜重和干重、DNA含量均随着Cr6+浓度的增加而降低。3d龄幼苗比10 d龄幼苗对Cr6+更敏感。琼脂糖凝胶电泳显示,对照根尖DNA带基本完整,而Cr6+处理的DNA则发生了降解,出现拖尾现象。[结论]Cr6+处理影响了小麦幼苗根系的正常生长并导致DNA含量下降。6+
译  名:
Research on the Root Grown of Wheat Seedling under Stress of Cr~(6+) and the Variation in Content of DNA
作  者:
HEI Shu-mei(College of Life Sciences,Yanan University,Yanan,Shaanxi 716000)
关键词:
Cr6+;Wheat seedling;Root;Content of DNA
摘  要:
[Objective] The effect of heavy metal-Cr6+ on the root growth of wheat seedling and the content of DNA.was analyzed.[Method] The growth index of 3——10 day-old wheat seedling,treated with Cr6+ with the concentration of 5-100 mg/L,respectively,was measured,and content of DNA was tested with the method of UV spectrophotometry and electrophoresis.[Result] Except the treatment of Cr6+ > 20 mg/L,in which,the number of root in 3 day-old wheat seedling was less than that of the CK,the number of root in other treatments was increased;and the length fresh weight and dry weight of root,the content of DNA were increased with the concentration-increasing of Cr6+.3 day-old seedling was more sensitive to Cr6+ than 10 day-old seedling.The analysis of agarose gel electrophoresis indicated that the DNA in root tip of CK was basic integrity and it was degraded in all of treatments',showing its smearing.[Conclusion] The treatment of Cr6+ affected the normal root growth of wheat seedling and led the content-decreasing of DNA.

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