当前位置: 首页 > 文章 > 铬胁迫对小麦幼苗叶片DNA含量的影响 安徽农业科学 2011,39 (16) 47-48
Position: Home > Articles > Effect of Cr~(6+) Stress on the DNA Content in Leaves of Wheat Seedlings Journal of Anhui Agricultural Sciences 2011,39 (16) 47-48

铬胁迫对小麦幼苗叶片DNA含量的影响

作  者:
黑淑梅
单  位:
延安大学生命科学学院
关键词:
Cr6+;小麦幼苗;DNA含量;电泳
摘  要:
[目的]研究Cr6+对小麦幼苗叶片DNA含量的影响。[方法]用5~100 mg/LCr6+分别处理3和10 d龄小麦幼苗,紫外分光光度法和电泳法测定DNA含量变化。[结果]Cr6+处理的小麦幼苗叶片DNA含量显著降低,3 d龄幼苗叶片DNA含量的下降幅度大于10 d龄幼苗。电泳图谱表明,对照叶片DNA带基本完整,而Cr6+处理的DNA则发生了降解,出现拖尾现象。[结论]Cr6+处理减少了小麦幼苗叶片DNA含量,进而影响其正常生长。6+
译  名:
Effect of Cr~(6+) Stress on the DNA Content in Leaves of Wheat Seedlings
作  者:
HEI Shu-mei(College of Life Science,Yan'an University,Yan'an,Shaanxi 716000)
关键词:
Cr6+;Wheat seedlings;DNA content;Agarose gel electrophoresis
摘  要:
[Objective] To study the effect of Cr6+ on the DNA content in leaves of wheat seedlings.[Method] 3-day-old and 10-day-old wheat seedlings were treated separately with 5-100 mg/L Cr6+,after that the DNA content was determined by UV spectrophotometry method and agarose gel electrophoresis.[Result] The content of DNA in leaves of Cr6+ treated wheat seedlings all reduced significantly,the decrease of DNA content in 3-day-old seedlings was bigger than that in 10-day-old seedlings.Agarose gel electrophoresis indicated that DNA belt was basic complete in leaf of control seedling,but the DNA belts in leaf of Cr6+ treated seedling had the degradation with the trailing phenomenon.[Conclusion] Cr6+ treatment led to the decrease of DNA content in leaves and affected the abnormal growth of wheat seedlings.

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