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当前位置: 首页 > 文章 > 高效液相色谱法测定酵母粉中硫胺素 安徽农业科学 2012,40 (17) 290-292
Position: Home > Articles > Determination of Thia mine Content in Yeast Powder by High Performance Liquid Chromatography Journal of Anhui Agricultural Sciences 2012,40 (17) 290-292

高效液相色谱法测定酵母粉中硫胺素

作  者：
纪龙翔;黄倩;张建璀;赵章锁;王玉娟;刘国生
单  位：
河南师范大学生命科学学院
关键词：
高效液相色谱;硫胺素;酵母粉;肌苷发酵
摘  要：
[目的]实现企业在肌苷发酵生产中对酵母粉原料质量的评价,为肌苷发酵的控制奠定重要的方法学基础。[方法]将酵母粉经过高温高压并用盐酸提取,通过试验确定高效液相色谱法分离条件并测定硫胺素含量。[结果]采用Hypersil BDS C18色谱柱,紫外检测波长为254 nm;以0.02 mol/L pH 3的磷酸盐缓冲液为流动相,流速1.0 ml/min对提取液进行色谱测定,可以将硫胺素与其他成分有效分离。用0.1 mol/L HCl悬浮后在超声清洗器中振荡10 min混合均匀,然后于121℃高温高压下30 min离心可将酵母粉中的硫胺素充分释放出来。[结论]采用高效液相色谱法测定酵母粉中硫胺素含量,该方法快速、准确,测定结果可做为评价酵母粉质量的指标,对肌苷发酵有重要指导意义。
译  名：
Determination of Thia mine Content in Yeast Powder by High Performance Liquid Chromatography
作  者：
JI Long-xiang et al(College of Life Sciences,Henan Normal University,Xinxiang,Henan 453007)
关键词：
HPLC;Thiamine;Yeast powder;Inosine fermentation
摘  要：
[Objective] To evaluate the quality of yeast powder for the industrialized production of inosine fermentation,so as to lay methodological basis for the control of inosine fermentation.[Method] After high temperature and high pressure treatment,the yeast powder was extracted with HCl,the HPLC conditions were determined through experiment and the content of thiamine was measured.[Result] The extraction liquid was chromatographically determined with Hypersil BDS C18 column at the UV detection wavelength of 254 nm and with 0.02 mol/L and pH 3 phosphate buffer as the mobile phases at a flow rate of 1.0 ml/min.The thiamine can be effectively separated from the other ingredients under the chromatographic conditions.The yeast extract which was suspended with 0.1 mol/L HCl was shocked by the ultrasonic cleaner for 10 min to mix well,and then centrifuged for 30 min at 121 ℃,thus the thiamine was fully extracted out.[Conclusion] The HPLC method is rapid and accurate,its determination results can be used to evaluate the quality of yeast powder and has great significance to inosine fermentation.

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