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当前位置: 首页 > 文章 > 2%宁南霉素水剂对烟草花叶病毒的抑制及作用机制的初步研究 农药 2008,47 (1) 37-40
Position: Home > Articles > Inhibition Activity and Mechanism Primary Study of Ningnanmycin 2% AS to Tobacco Mosaic Virus Agrochemicals 2008,47 (1) 37-40

2%宁南霉素水剂对烟草花叶病毒的抑制及作用机制的初步研究

作  者：
蔡学建;陈卓;宋宝安;杨松;胡德禹;薛伟;严凯
单  位：
贵州大学精细化工研究开发中心教育部绿色农药与生物工程重点实验室
关键词：
宁南霉素;烟草花叶病毒;防御酶;病程相关蛋白基因;基因表达;抑制机制
摘  要：
采用半叶法测定2%宁南霉素水剂(AS)在500mg/L下抗烟草花叶病毒(TMV)的活体治疗、保护及钝化活性,检测2%宁南霉素AS诱导烟叶中内防御酶活性变化及病程相关蛋白基因的表达。结果表明,2%宁南霉素AS具有较高的抗TMV活性,同时2%宁南霉素AS处理可导致接种TMV烟草品种K326叶片的PAL、POD、SOD活性不同程度提高和PR-1a、PR-5基因表达上调,表明2%宁南霉素AS能启动烟草的一系列抗病防御反应,诱导烟草产生抗病性。
译  名：
Inhibition Activity and Mechanism Primary Study of Ningnanmycin 2% AS to Tobacco Mosaic Virus
作  者：
CAI Xue-jian,CHEN Zhuo,SONG Bao-an,YANG Song,HU De-yu,XUE Wei,YAN Kai (Key Laboratory of Green Pesticide and Agricultural Bioengineering,Ministry of Education,Center for Research and Development for Fine Chemicals,Guizhou University,Guiyang 550025,China)
关键词：
ningnamycin;tobacco mosaic virus;defensive enzyme;pathogenesis related proteins gene;gene expression; inhibition mechanism
摘  要：
A half-leaf method was used to determine protective,curative,and inactivation efficacies of ningnanmycin against tobacco mosaic virus(TMV)at 500 mg/L in vivo.The activity of defensive enzymes and expression of pathogenesis-related proteins genes were also tested.Bioassay performed by conventional half-leaf method revealed that ningnanmycin showed highly inhibitory activity against TMV at a concentration of 500 mg/L.The activity of PAL,POD,SOD of tobacco K326 leaves was found to increase in different proportions after being treated with ningnanmycin,which also induced up-regulation of PR-1a,PR-5 genes expression.The result suggests that ningnanmycin stimulate defense reaction of tobacco and induce the disease resistance of tobacco.

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