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Position: Home > Articles > Rapid detection of docosapentenoic acid and docosahexaenoic acid in algae oil by ultra performance liquid chromatography Science and Technology of Food Industry 2014,35 (9) 278-281

超高效液相色谱法测定藻油中的DPA和DHA

作  者：
晋文慧;陈伟珠;洪专;张怡评;方华;谢全灵
单  位：
国家海洋局第三海洋研究所国家海洋局海洋生物资源综合利用工程技术研究中心
关键词：
超高效液相色谱(UPLC);二十二碳五烯酸甲酯(DPA-ME);二十二碳六烯酸甲酯(DHA-ME);藻油
摘  要：
采用超高效液相色谱(UPLC)方法快速分析测定藻油中DPA甲酯和DHA甲酯的含量,考察了流动相、流速及柱温等条件对藻油中DPA-ME和DHA-ME分离的影响,确定了最佳色谱条件:等度洗脱,流动相为乙腈-水(80∶20,V/V),流速0.5mL/min,检测波长205nm。该方法下,二十二碳五烯酸甲酯(DPA-ME)和二十二碳六烯酸甲酯(DHA-ME)分别在10.2~510.0、11.1~554.0μg/mL范围内浓度和峰面积呈现良好的线性关系,相关系数r均为0.9999;平均回收率分别为99.1%(RSD=1.3%)和100.6%(RSD=1.4%)。本方法快速、灵敏、重复性好,适合于藻油中DPA和DHA含量的检测。
译  名：
Rapid detection of docosapentenoic acid and docosahexaenoic acid in algae oil by ultra performance liquid chromatography
作  者：
JIN Wen-hui;CHEN Wei-zhu;HONG Zhuan;ZHANG Yi-ping;FANG Hua;XIE Quan-ling;Engineering Research Center of Marine Biological Resource Comprehensive Utilization,SOA,The Third Institute of Oceanography of the State Oceanic Administration;
关键词：
ultra performance liquid chromatography(UPLC);;docosapentenoic acid methyl ester(DPA-ME);;docosahexaenoic acid methyl ester(DHA-ME);;algae oil
摘  要：
In order to determine the content of docosapentenoic acid( DPA) and docosahexaenoic acid( DHA) in algae oil quickly and efficiently,a rapid ultra- high pressure liquid chromatography( UPLC) method was developed.The influence of mobile phase,flow rate on the separation of DPA- ME and DHA- ME in algae oil were comprehensively studied.The optimal separation conditions were as follows: a mobile phase consisting of acetonitrile and water( 80∶20,V/V) with a isocratic elution profile,UV detection wavelength at 205nm,0.5mL /min of flow rate for mobile phase.On the above conditions,good linearity between response( peak area) and concentration was found over a concentration range of 10.2~510.0μg /mL for DPA- ME,and 11.1 ~554.0μg /mL for DHA- ME respectively.And the correlation coefficient of the calibration curve of DPA- ME and DHA- ME was 0.9999.The average recovery rates of DPA-ME and DHA-ME were 99.1%( RSD = 1.3%) and 100.6%( RSD = 1.4%) respectively.This method was high- speed,sensitive and with a good reproducibility.It was appropriate for fast detection of DPA and DHA in algae oil.

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