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当前位置: 首页 > 文章 > 小麦叶片衰老态势核磁共振分析 农业机械学报 2014,45 (4) 269-275
Position: Home > Articles > Investigating Senescence Status of Wheat Leaves by Nuclear Magnetic Resonance Transactions of the Chinese Society for Agricultural Machinery 2014,45 (4) 269-275

小麦叶片衰老态势核磁共振分析

作  者：
杜光源;唐燕;张嵩午;王长发;郭蔼光
单  位：
西北农林科技大学农学院;西北农林科技大学理学院;西北农林科技大学生命科学学院
关键词：
小麦叶片;衰老;核磁共振;T2弛豫时间;磁共振成像
摘  要：
建立了小麦的核磁共振活体检测体系,通过监测小麦叶片的T2弛豫特性、含水率以及叶绿素含量在灌浆中后期的日序变化,发现从叶片衰老初期至降解期,T2弛豫谱幅度和含水率变化不明显,平均T2弛豫时间持续增大,而叶绿素含量逐渐减小;到叶片衰亡期,T2弛豫谱幅度、含水率、平均T2弛豫时间以及叶绿素含量均迅速减小至最小值。由此可见,小麦叶片的T2弛豫谱幅度与含水率具有正相关关系,而平均T2弛豫时间与生理活性有关。最后根据叶片的T2弛豫特性,分析了核磁共振T2弛豫谱、磁共振T1加权成像以及T2加权成像等方法在评价小麦叶片衰老态势方面的特点。
译  名：
Investigating Senescence Status of Wheat Leaves by Nuclear Magnetic Resonance
作  者：
Du Guangyuan;Tang Yan;Zhang Songwu;Wang Changfa;Guo Aiguang;College of Science,Northwest A&F University;College of Life Sciences,Northwest A&F University;College of Agronomy,Northwest A&F University;
关键词：
Wheat leaf Senescence Nuclear magnetic resonance T2relaxation time Magnetic resonance imaging
摘  要：
Nuclear magnetic resonance(NMR) technique has been broadly used in probing plant water relations.However,the relationship between NMR parameters and photosynthetic traits is still not clear.An NMR system was constructed to perform non-imaging relaxation measurement or magnetic resonance imaging on wheat non-invasively and non-destructively.It was found that from the onset of leaf senescence to the degradation stage,the average T2relaxation time(T2,mean) increased gradually and the chlorophyll content decreased,while the amplitude(A) of T2relaxation spectrum and water content kept stable.When wheat leaves approached cell death,all of the above mentioned NMR and physiological parameters decreased to its minimum value.It was deduced that A and the water content of wheat leaves were linearly correlated.On the other hand,T2,meanwas related with leaf senescence status.Therefore, non-imaging T2relaxation technique was thought a fast and powerful method to estimate the senescence status of wheat leaf.In addition,T1weighted imaging and T2weighted imaging could represent leaf physiological status directly based on the relaxation property.T1weighted imaging was comparatively time-saving,whereas T2weighted imaging was much more sensitive to leaf aging.

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