当前位置: 首页 > 文章 > 种衣剂对大豆根际固氮菌多样性的影响 中国油料作物学报 2010,32 (1) 132-135
Position: Home > Articles > Impact of seed coatings on diversity of soybean rhizospheric azotobacter Chinese Journal of Oil Crop Sciences 2010,32 (1) 132-135

种衣剂对大豆根际固氮菌多样性的影响

作  者:
韩雪;孙龙;仕相林;尹晓玉;文景芝
单  位:
东北农业大学农学院
关键词:
固氮菌;nifH基因;多样性;PCR-DGGE
摘  要:
利用变性梯度凝胶电泳(DGGE)技术研究宁南霉素、甲霜灵、多克福和咯菌腈4种种衣剂对大豆根际固氮菌多样性的影响。运用nifH基因的特异引物对,将大豆根际土壤总DNA进行PCR扩增后,通过DGGE技术对PCR产物进行分析。结果表明,种衣剂对固氮菌产生了不同程度的抑制作用。对大豆根际固氮菌抑制作用由强到弱的种衣剂品种依次是咯菌腈、甲霜灵、多克福和宁南霉素;种衣剂浓度对大豆根际固氮菌多样性的抑制程度不同,但没有明显规律。
译  名:
Impact of seed coatings on diversity of soybean rhizospheric azotobacter
作  者:
HAN Xue,SUN Long,SHI Xiang-lin,YIN Xiao-yu,WEN Jing-zhi(Department of Plant Protection,Agricultural College,Northeast Agricultural University,Harbin 150030,China)
关键词:
Azotobacter;nif H gene;Diversity;PCR-DGGE
摘  要:
The impact of different seed coatings on diversity of soybean rhizospheric azotobacter was analyzed by detection of nif H gene diversity using denaturing gradient gel electrophoresis (DGGE). The results indicated that different kinds of seed coatings were suppressive to soybean rhizospheric azotobacter. The suppressive effect in a decreasing order was Fludioxonil,Metalaxyl,Carbofuran-Carbendazim-Thiram and Ninanmycin. The suppressive effects varied with the concentration of seed coatings without clear regularity.

相似文章

计量
文章访问数: 14
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊