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Position: Home > Articles > Purification of Akabane Disease Virus and Preparation of Its Monoclonal Antibody China Animal Health Inspection 2009,26 (4) 29-31

赤羽病病毒的纯化及其单克隆抗体的制备

作  者:
赵样平;屈浩;董志珍;肖妍;侯艳梅;郭桂庆;黄丽华;王冬梅;张宇光
单  位:
协和干细胞基因工程有限公司;天津出入境检验检疫局
关键词:
赤羽病病毒;纯化;单克隆抗体
摘  要:
将人工繁殖、纯化的赤羽病病毒作为免疫原,通过常规杂交瘤技术,制备亲和性强、特异性好的小鼠抗赤羽病病毒单克隆抗体,并进行相应的纯化和标记工作。以澳大利亚进口的试剂盒进行验证,结果证明:进口单抗与我们制备的AAK纯品及AAK-HRP显示同样结果;且质控结果表明,AAK纯品及其衍生物AAK-HRP的特异性和亲和性均基本满足进行封闭ELISA检测方法的要求,为制备国产化ELISA试剂盒提供了基础。
译  名:
Purification of Akabane Disease Virus and Preparation of Its Monoclonal Antibody
作  者:
Zhao Xiang-ping1,Qu Hao2,Dong Zhizhen1,Zhao Yan1,Hou Yanmei1, Guo Guiqing2,Huang Lihua2,Wang DongMei2, Zhang Yuguang2 (1.TianJin Exited Entry Inspection and Quarantine Bureau;2.Xiehe stem cell Gene Engineering Co. Ltd.)
关键词:
Akabane Disease Virus; Purification; Monoclonal Antibody
摘  要:
A monoclonal antibody against Akabane virus was developed using artificial grown virus. Normal hybridoma technology was used to prepare more specific and sensitive mouse anti-Akabane virus monoclonal anti-body, which was then purified and labeled, and was compared with the Australia commercial Elisa-kit . It was certified that our AAK and AAK-HRP had the same effect as the imported monoclonal antibody kit, and from the quality control results, both specificity and sensibility of AAK or AAK-HRP met requirements for ELISA. The study provides the basis for the developrent of domestic ELISA kit for akabane disease virus.

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