中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > SIMYB1 and SIMYB2, two new MYB genes from tomato, transcriptionally regulate cellulose biosynthesis in tobacco 农业科学学报 (英文) 2017,16 (1)
Position: Home > Articles > SIMYB1 and SIMYB2, two new MYB genes from tomato, transcriptionally regulate cellulose biosynthesis in tobacco Journal of Integrative Agriculture 2017,16 (1)

SIMYB1 and SIMYB2, two new MYB genes from tomato, transcriptionally regulate cellulose biosynthesis in tobacco

作  者：
Shi Yan-na;Liu Xiao-fen;Li Xue;Dong Wen-cheng;Grierson, Donald;Yin Xue-ren;Chen Kun-song
单  位：
Univ Nottingham, Sch Biosci, Plant & Crop Sci Div, Sutton Bonington Campus, Loughborough LE12 5RD, Leics, England;Zhejiang Univ, Lab Hort Plant Growth Dev & Qual Improvement, Minist Agr, Hangzhou 310058, Zhejiang, Peoples R China;Zhejiang Univ, Zhejiang Prov Key Lab Hort Plant Integrat Biol, Hangzhou 310058, Zhejiang, Peoples R China;Zhejiang Univ, Coll Agr & Biotechnol, Hangzhou 310058, Zhejiang, Peoples R China
关键词：
MYB;cellulose;cellulose synthase;transcriptional regulation;tomato;tobacco
摘  要：
Cellulose, a major constituent of plant biomass, is synthesized by a cellulose synthase complex. It has been demonstrated that MYB genes transcriptionally regulate cellulose synthase in Arabidopsis. However, little is known about this process in tomato. Here, two MYB (SIMYB1/2) and three cellulose synthase (CESA)(SICESA4/5/6) genes were isolated. SIMYB1/2 and SICESA4/5/6 accumulation was found to correspond to cellulose accumulation in different tissues of tomato. Dual luciferase assays indicated that these two MYBs were transcriptional activators that interact with promoters of SICESA4/5/6. Moreover, SIMYB2 could also activate promoters of SIMYBI/2, suggesting the possible underlying auto-activation mechanisms for MYB transcription factors. Transient over-expression of SIMYB1/2 in Nicotiana tebacum up-regulated tobacco endogenous NtCESA genes and increased cellulose accumulation. The function of SIMYB1/2 was further investigated using stable transformation and the results indicated that N. tabacum lines heterologous expressing SIMYBI/2 displayed a pleiotropic phenotype, long and narrow leaves, with NtCESA induced and significant increase of cellulose. In conclusion, our data suggest that tomato SIMYBI/2 have transcriptional regulatory roles in cellulose biosynthesis and SIMYB2 was more effective than SIMYBI, which may due to the transcriptional activation by SIMYB2 on SIMYBI and itself.

相似文章

导出引用
计量
文章访问数: 15
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46