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当前位置: 首页 > 文章 > 玉米短链脱氢酶基因IDP2557的克隆及其抗旱功能鉴定 分子植物育种 2019 (22) 7300-7305
Position: Home > Articles > Cloning of IDP2557 Gene Encoding Maize Short-chain Dehydrogenase and Identification of Its Drought Resistance Molecular Plant Breeding 2019 (22) 7300-7305

玉米短链脱氢酶基因IDP2557的克隆及其抗旱功能鉴定

作  者：
崔扬;冯彦辉;陈众峰;王晓敏;王云鹏;杜贤章
单  位：
吉林省鸿翔农业集团鸿翔种业有限公司;吉林省农业科学院农业生物技术研究所植物生物反应器工程实验室吉林省农业生物技术重点实验室
关键词：
玉米(Zea mays);短链脱氢酶;zmIDP2557;干旱胁迫
摘  要：
短链脱氢酶是分布最为广泛的一类氧化还原酶,并通过参与各种初生代谢及次生代谢反应调节动植物、微生物的生理生化反应。早期工作中发现耐旱玉米品系玉米自选系X923-1根系中zmIDP2557基因受干旱胁迫特异性诱导表达,推测其可能与玉米抗旱胁迫相关。为鉴定其在干旱胁迫下的生物学功能,我们通过RT-PCR的方法从耐旱玉米自选系X923-1根部总RNA中克隆了该基因。该基因包含一个996 bp的开放阅读框,其编码的氨基酸序列与B73相比,有3个位点发生了突变,推测其可能与其抗旱能力的产生相关。构建该基因由组成型启动子35S驱动植物表达载体,并通过农杆菌介导法获得该基因过表达的转基因玉米植株,通过分子检测证实该基因能够稳定遗传并正常转录。最后通过对T1代转基因玉米进行抗旱性鉴定,证实过表达该基因能够显著提高转基因玉米的抗旱能力,zmIDP2557基因是玉米耐受干旱胁迫的优异基因资源。对该基因的功能解析与抗性研究工作可为利用其进行玉米抗旱分子育种工作提供理论支持。
译  名：
Cloning of IDP2557 Gene Encoding Maize Short-chain Dehydrogenase and Identification of Its Drought Resistance
作  者：
Cui Yang;Feng Yanhui;Chen Zhongfeng;Wang Xiaomin;Wang Yunpeng;Du Xianzhang;Jilin Hongxiang Agriculture Corporation Hongxiang Seeding Limited Company;Jilin Provincial Key Laboratory of Agricultural Biotechnology, Laboratory of Plant Bioreactor Engineering, Institute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences;
关键词：
Maize(Zea mays);;Short-chain dehydrogenase;;zmIDP2557;;Drought stress
摘  要：
Short-chain dehydrogenase is the most widely distributed class of oxidoreductases, and regulates physiological responses and biochemical reactions in plants, animals and microorganisms by participating in various primary and secondary metabolic reactions. In the early work, zmIDP2557, a short-chain dehydrogenase gene, in a drought-tolerant maize line, X923-1, was found specifically induced by drought stress, which may be related to drought resistance in maize. To identify its biological function under drought stress, we cloned the gene from total RNA of the drought-tolerant maize line X923-1 by RT-PCR method. The gene contains a 996 bp open reading frame, and encodes an amino acid sequence with mutations at three sites referenced to the maize B73 genome, these mutation are suggested to be related to its ability to against drought stress. A plant expression vector was constructed with the constitutive promoter 35 S to drive zmIDP2557, and zmIDP2557 overexpressing transgenic maize plants were obtained by Agrobacterium-mediated transformation. The molecular analysis confirmed that the transgene could be stably inherited and normally transcripted. Drought resistance assessment of T1 transgenic maize was eventually performed and it was proved that overexpression of zmIDP2557 could significantly improve the drought resistance of transgenic maize. These results suggested that zmIDP2557 can be used as an excellent genetic resource for maize tolerance to drought stress. The functional analysis of this gene can provide theoretical support for the use of maize breeding for drought resistance.

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