当前位置: 首页 > 文章 > 不同浓度激素对省沽油组培过程中芽增殖影响的研究 安徽农业科学 2013 (29) 11718-11719
Position: Home > Articles > Effects of Different Concentration Hormones on Bud Proliferation in Tissue Culture of Staphylea bumalda Journal of Anhui Agricultural Sciences 2013 (29) 11718-11719

不同浓度激素对省沽油组培过程中芽增殖影响的研究

作  者:
陶许一;邵庆;毕璋友
单  位:
安庆职业技术学院
关键词:
省沽油;6-BA;IAA;浓度;芽增殖;生芽率
摘  要:
[目的]了解不同浓度激素下对省沽油组培芽增殖的影响。[方法]研究了常用浓度区间内的6-BA以及6-BA、IAA混用对于省沽油外植体生芽率的影响。[结果]当6-BA浓度为0.6 mg/ml时,省沽油茎段在接种后第15 d生芽率达到84%;6-BA浓度在0.4 mg/ml时,接种后10 d的茎尖生芽率达到56%;1 mg/ml 6-BA+0.20 mg/ml IAA浓度茎尖生芽率达到50%,茎段生芽率达到30%。[结论]省沽油组培芽增殖试验中,单独使用0.4~0.6 mg/ml的6-BA可达到最好的生芽刺激效果。
译  名:
Effects of Different Concentration Hormones on Bud Proliferation in Tissue Culture of Staphylea bumalda
作  者:
TAO Xu-yi;Anqing Vocational and Technical College;
关键词:
Staphylea bumalda DC.;;6-BA;;IAA;;Concentration;;Bud proliferation;;Budding rate
摘  要:
[Objective] To study effects of different concentration hormones on bud proliferation in tissue culture of Staphylea bumalda DC. [Method]The effects of 6-BA in commonly used concentration range and 6-BA,IAA mixture on explant germination rate of S. bumalda were studied. [Result] When 6-BA concentration came to 0. 6 mg / ml,S. bumalda stem section germination rate reach a percentage of 84 at the 15th day; with the 6-BA at the concentration of 0. 4 mg / ml,we saw a stem germination rate of 56% on the 10th day; when the IAA concentration was set on 0. 20 mg / ml,with a invariant percentage of 6-BA,the rata jumped up to 50%,stem section germination rate came to 30%. [Conclusion]In the test of the bud proliferation of S. bumalda,an exclusive use of 6-BA at 0.4-0.6 mg/ml proved to exert the most significant stimulus effect.

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