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Position: Home > Articles > Research on Explants Sterilization Technique in Staphylea bumalda DC.Tissue Culture Process Journal of Anhui Agricultural Sciences 2012,40 (4) 2110+2208

省沽油组培过程中外植体灭菌技术的研究

作  者:
朱秀蕾;毕璋友;饶敏
单  位:
安庆职业技术学院园林园艺系
关键词:
省沽油;组织培养;外植体;灭菌技术
摘  要:
[目的]寻求省沽油组织培养中适宜的外植体灭菌方法。[方法]研究了70%乙醇、0.1%HgCl2、0.1%HgCl2+70%乙醇组合在不同灭菌时间内对外植体生长的影响。[结果]适宜的外植体灭菌技术:茎尖用1%HgCl2+70%乙醇处理时间分别为5 min+50 s,成活率100%;茎段用1%HgCl2处理时间为15 m,成活率95%;幼叶用1%HgCl2处理时间为12 min,成活率85%。[结论]1%HgCl2+70%乙醇、1%HgCl2是省沽油外植体的有效灭菌剂。
译  名:
Research on Explants Sterilization Technique in Staphylea bumalda DC.Tissue Culture Process
作  者:
ZHU Xiu-lei et al(Anqing Vocational and Technical College,Department of Horticulture and Landscape,Anqing,Anhui 246003)
关键词:
Staphylea bumalda DC.;Tissue culture;Explants;Sterilization
摘  要:
[Objective] The aim was to seek appropriate explants sterilization method in Staphylea bumalda DC.tissue culture process.[Method] The comparative method was used to study the effect of alcohol,HgCl2,HgCl2+alcohol combination in different sterilization time on explants growth.[Result] The results showed that the appropriate explant sterilization technology was the shoot tips survival rate was 100% with 1% HgCl2+70% alcohol,processing time was 5 min+50 s respectively;The stem sections survival rate was 95% with 1% HgCl2,processing time was 15 min;The young leaves survival rate was 85% with 1% HgCl2,processing time was 12 min.[Conclusion] 1% HgCl2+70% alcohol,1% HgCl2 was effective sterilant of Staphylea bumalda DC.explants.

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