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当前位置: 首页 > 文章 > 化学发光微粒子免疫法检测虾肉中磺胺类药物的残留 安徽农业科学 2018 (8) 170-173
Position: Home > Articles > Detection of Sulfonamides Residues in Shrimp by Chemiluminescence Microparticle Immuno Assay (CMIA) Journal of Anhui Agricultural Sciences 2018 (8) 170-173

化学发光微粒子免疫法检测虾肉中磺胺类药物的残留

作  者：
汪善良;程茹;钟新敏;张凯;谢体波;李平;吴紫洁;袁光宇;陈吉香
单  位：
贵州勤邦食品安全科学技术有限公司
关键词：
虾肉;化学发光微粒子免疫法;高效液相色谱-质谱法;磺胺类药物
摘  要：
[目的]通过对比化学发光微粒子免疫分析法(CMIA)和高效液相色谱-质谱法对虾肉中磺胺类药物检测结果的差异,验证公司自研的磺胺类药物化学发光免疫试剂盒的检测效果。[方法]以虾肉为检测原料,采用直接竞争CMIA法对试剂盒进行灵敏度、特异性、精密度、准确度试验。[结果]试剂盒检测灵敏度为1.04μg/kg、加样回收率为95.25%~104.50%,变异系数(CV)均小于15%;试剂盒检测结果与仪器分析的阴、阳性结果接近。[结论]该方法检测结果稳定、可靠,达到国家相关标准,表明该款试剂盒可满足动物组织中磺胺类药物的快速检测需求。
译  名：
Detection of Sulfonamides Residues in Shrimp by Chemiluminescence Microparticle Immuno Assay (CMIA)
作  者：
WANG Shan-liang;CHENG Ru;ZHONG Xin-min;Guizhou Kwinbon Food Safety Science-Technology Co.,Ltd.;
关键词：
Shrimp;;Chemiluminescent microparticle immuno assay (CMIA);;High performance liquid chromaloagraphy-mass spectrometry (HPLC-MS);;Sulfonamides
摘  要：
[Objective]To verify the test effect of the company's production of sulfonamides chemiluminescence microparticle immuno assay( CMIA) detection kit,the method of chemiluminescent microparticle immuno assay and high performance liquid chromatography-mass spectrometry was used to detect the residues of sulfonamides in shrimp samples,and compared the difference between the two methods of test results. [Method]Shrimp meat as raw material for detection,the kits about sensitivity,specificity,precision and accuracy were tested by direct competitive CMIA method. [Result]The sensitivity of the kit was 1. 04 μg/kg,the average recovery was 95. 25%-104. 50%,the coefficient of variation CV all were less than 15%; The results for shrimp by CMIA method were well consistent with as the results of HPLC-MS method.[Conclusion]The results for shrimp by CMIA method are stable and reliable,and meet the relevant national standards. It shows that this kit can meet the requirements of rapid detection of sulfonamides in animal tissues.

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