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当前位置: 首页 > 文章 > 山楂黄酮对小鼠脾淋巴细胞的免疫调节作用 食品工业科技 2019,40 (20) 127-132+145
Position: Home > Articles > Immunoregulatory Effect of Hawthorn Flavonoids on Spleen Lymphocytes in Mice Science and Technology of Food Industry 2019,40 (20) 127-132+145

山楂黄酮对小鼠脾淋巴细胞的免疫调节作用

作  者：
王一伦;李敬双;李美莹;于洋
单  位：
锦州医科大学食品科学与工程学院
关键词：
山楂黄酮;淋巴细胞增殖;细胞因子;淋巴细胞膜表面标志;免疫调节
摘  要：
为研究山楂黄酮对小鼠脾淋巴细胞的免疫调节作用,将小鼠分为空白组、左旋咪唑组和不同浓度山楂黄酮处理组(100、200、400和800μg/mL)。利用MTT法检测山楂黄酮对淋巴细胞增殖的影响,ELISA法检测山楂黄酮对淋巴细胞白细胞介素-6(IL-6)、白细胞介素-4(IL-4)、干扰素-γ(IFN-γ)的影响,流式细胞术检测山楂黄酮对淋巴细胞膜表面标志物(CD4~+和CD8~+)的影响。结果表明,山楂黄酮在100～400μg/mL对淋巴细胞增殖和淋巴细胞因子的分泌具有浓度依赖性,IL-6和IFN-γ的分泌极显著高于空白组(P <0.01),IL-4的分泌极显著低于空白组(P <0.01),且400μg/mL处理组与左旋咪唑组无统计学意义(P> 0.05);对淋巴细胞亚群CD4~+CD8~-、CD4~-CD8~+具有浓度依赖性的促进作用,且极显著高于空白组、左旋咪唑组(P <0.01);对淋巴细胞亚群CD4~+/CD8~+百分比极显著高于空白组、左旋咪唑组(P <0.01),200μg/mL浓度组CD4~+/CD8~+的比值极显著高于其它浓度组(P <0.01)。100～400μg/mL山楂黄酮通过诱导淋巴细胞增殖和淋巴细胞因子IL-6、IL-4和IFN-γ的分泌,提高淋巴细胞亚群CD4~+/CD8~+比值,从而发挥免疫调节作用。
译  名：
Immunoregulatory Effect of Hawthorn Flavonoids on Spleen Lymphocytes in Mice
作  者：
WANG Yi-lun;LI Jing-shuang;LI Mei-ying;YU Yang;College of Food Science and Engineering,Jinzhou Medical University;
关键词：
hawthorn flavonoids;;lymphocyte proliferation;;cytokines;;surface marker of lymphocyte membrane;;immunoregulation
摘  要：
To study the immunomodulatory effect of hawthorn flavonoids on spleen lymphocytes in mice,mice were divided into blank group,levamisole group and hawthorn flavonoids treatment group( 100,200,400 and 800 μg/mL).MTT method was used to detect the effect of hawthorn flavonoids on lymphocyte proliferation.ELISA method was used to detect the effect of hawthorn flavonoids on lymphocyte interleukin-6( IL-6),interleukin-4( IL-4),interferon-γ( IFN-γ). Flow cytometry was used to detect the effect of hawthorn flavonoids on lymphocyte membrane surface markers( CD4~+and CD8~+). Results showed that hawthorn flavonoids had concentration dependence on lymphocyte proliferation and lymphocyte factor secretion ranged from 100 to 400 μg/mL.The secretion of IL-6 and IFN-γ were higher than that of the blank group extremely significant( P < 0.01),and the secretion of IL-4 was lower than that of the blank group extremely significant( P < 0.01),and there was no statistical significance between the 400 μg/mL treatment group and levamisole group( P > 0.05). The percentage of CD4~+CD8~-and CD4~-CD8~+ in lymphocyte subsets had a centration-dependent promoting effect,which was higher than that in blank group and levamisole group extremely significant( P < 0.01).The percentage of CD4~+/CD8~+in lymphocyte subsets was higher than that in blank group and levamisole group extremely significant( P < 0.01),and the ratio of CD4~+/CD8~+ in 200 μg/mL treatment group was higher than that in other concentration groups( P < 0.01). Hawthorn flavone could enhance the CD4~+/CD8~+ ratio of lymphocyte subpopulation by inducing lymphocyte proliferation and secretion of lymphocyte factors IL-6,IL-4 and IFN-γ ranged from 100 to 400 μg/mL,and it played an immunomodulatory role.

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