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当前位置: 首页 > 文章 > 猪瘟病毒与猪流行性乙型脑炎病毒复合RT-PCR检测方法的建立 西北农业学报 2009,18 (3) 14-17
Position: Home > Articles > Multiplex RT-PCR for Detection of Classical Swine Fever Virus and Japanese Encephalitis Virus Acta Agriculturae Boreali-occidentalis Sinica 2009,18 (3) 14-17

猪瘟病毒与猪流行性乙型脑炎病毒复合RT-PCR检测方法的建立

作  者：
薛媛;杨柳絮;张淼涛
单  位：
西北农林科技大学动物医学院
关键词：
复合RT-PCR;CSFV;JEV
摘  要：
猪瘟病毒(CSFV)和流行性乙型脑炎病毒(JEV)是引起种猪繁殖障碍的病原,而且经常混合感染,及时准确诊断是防治的前提。根据CSFV的E2基因保守序列和JEV的E基因保守序列,选取2对引物建立检测CSFV、JEV的复合RT-PCR方法,分别扩增出了大小为508和1 015 bp特异性基因片段。应用此方法检测了10份临床样品(淋巴结、脾、肝、肺等组织),结果4份CSFV阳性,3份JEV阳性,其余为阴性。结果表明此CSFV和JEV复合RT-PCR诊断方法,具有很好的特异性和敏感性,可用于临床检测。
译  名：
Multiplex RT-PCR for Detection of Classical Swine Fever Virus and Japanese Encephalitis Virus
作  者：
XUE Yuan,YANG Liuxu and ZHANG Miaotao*(College of Veterinary Medicine,Northwest A&F Univercity,Yangling Shaanxi 712100,China)
关键词：
Multiplex RT-PCR;CSFV;JEV
摘  要：
Classical swine fever virus(CSFV) and Japanese encephalitis virus(JEV) are the major causes of some serious reproductive diseases and co-infection is common.Therefore rapid and reliable diagnosis is crucial for effective prevention and treatment.In this study,a multiplex RT-PCR was developed using two-pairs primers designed according to conserved sequences in E2 fragments of CSFV and that in E fragments of JEV.PCR products of 508bp and 1015bp were obtained for respective viruses.Ten organ samples(lung,lumph,liver,spleen,etal) were respectively detected by the multiplex RT-PCR,out of which four were CSFV positive,three were JEV positive,the control samples were negative.The results showed that the multiplex RT-PCR of CSFV and JEV was a rapidly specific and sensitive diagnostic method.

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