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Loss of function of <i>CENH3</i> causes genome instability in soybean

作  者:
Jing Wang;Xiaofei Wang;Wei‐Cai Yang;Hong-Ju L
单  位:
Center for Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; Center for Molecular Agrobiology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, Chin
关键词:
italic;cenh3;soybean;lt;chromosome elimination;centromere
摘  要:
Centromeres are the site where kinetochores assemble and spindle microtubules anchor to the chromosome during cell division. Centromeres are epigenetically specified by the centromeric histone H3 (CENH3). The imbalance of CENH3 loading rate or dosage on parental centromeres often leads to uniparental chromosome elimination in the offspring. A body of studies of CENH3 in genome stability have been reported in Arabidopsis, cotton, and many other monocots, but not in soybean (Glycine max), an important dicot crop. In our study, we identified a single-copy functional CENH3 in soybean and found its role in genome stability and parent-of-origin effect caused by the mutation of a conserved glycine site and parental genetic background. This study provide evidence that knockout of CENH3 in soybean has the potential to induce chromosome elimination and would shed light on the future development of CENH3-based haploid induction (HI) system and centromere biology in soybean.

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