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当前位置: 首页 > 文章 > 产脂肪酶菌株的筛选及其脂肪酶的双水相分离 湖北农业科学 2010,49 (2) 433-435
Position: Home > Articles > Screening of Lipase-producing Microorganism and Lipase Extraction by Two Aqueous Two-phase Systems Hubei Agricultural Sciences 2010,49 (2) 433-435

产脂肪酶菌株的筛选及其脂肪酶的双水相分离

作  者：
陈利梅;李德茂
单  位：
聊城大学农学院食品科学与工程系
关键词：
脂肪酶;发酵;酶活;双水相
摘  要：
从土壤中筛选到了两株产脂肪酶的菌株真菌A和真菌B。测定二者酶活,并对真菌B所产脂肪酶的双水相萃取体系进行了优化。结果表明,真菌A酶活为26.5U/mL,真菌B酶活为15.0U/mL,而真菌A比酶活为6618.62U/g,真菌B比酶活为7332.20U/g,选取真菌B为研究对象。最佳PEG分子量为2000,最佳PEG浓度为200g/L,最佳硫酸铵浓度为250g/L。
译  名：
Screening of Lipase-producing Microorganism and Lipase Extraction by Two Aqueous Two-phase Systems
作  者：
CHEN Li-mei,LI De-mao (Department of Food Science and Engineering,Liaocheng University,Liaocheng 252059,Shandong,China)
关键词：
lipase;fermentation;enzyme activity;aqueous two-phase
摘  要：
Two lipase-producing strains,named fungus A and B,were isolated from soil samples.The enzyme activities and specific activities of fungus A and B were determined.The results indicated that the lipase activity of A was higher than that of B (The lipase activity of A was 26.5U and B was 15.0U).And the specific activity of B was higher than that of A (The specific activity of A was 6 618.62 U /g and B was 7 332.20 U /g).As a result,fungus B was selected to optimize the aqueous two-phase system.The optimal conditions of extraction showed as follows:the molecular weight of PEG was 2 000,the concentration of PEG was 25%,and the concentration of (NH4)2SO4 was 20%.

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