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当前位置: 首页 > 文章 > 去甲肾上腺素对大肠杆菌耐药质粒接合转移的影响 南京农业大学学报 2017 (5) 901-908
Position: Home > Articles > Influence of norepinephrine on resistant plasmids conjugational transfer between Escherichia coli Journal of Nanjing Agricultural University 2017 (5) 901-908

去甲肾上腺素对大肠杆菌耐药质粒接合转移的影响

作  者：
翁梦薇;计徐;郑卫江;姚文
单  位：
南京农业大学动物科技学院
关键词：
去甲肾上腺素;大肠杆菌;耐药性;接合转移率
摘  要：
[目的]本研究旨在探讨生产中普遍存在的急性应激因素对细菌耐药基因水平转移的可能影响。[方法]选取分离自断奶香猪粪便的2株喹诺酮类敏感型大肠杆菌作为试验菌株,通过测定D600值比较菌株在添加不同浓度去甲肾上腺素培养液中的生长曲线,确定去甲肾上腺素对于大肠杆菌的最佳促生长浓度;分离自断奶香猪粪便的7株携带质粒喹诺酮类耐药基因(aac(6')-lb-cr、oqx AB、qnr S)的大肠杆菌,采用滤膜接合法,计算去甲肾上腺素诱导和未诱导的大肠杆菌分离株(供体菌)与大肠杆菌J53(受体菌)的接合转移率;PCR检测接合子及供、受体菌质粒介导抗性基因的携带情况;采用K-B纸片扩散法和琼脂稀释法测定接合子及供、受体菌的多种抗生素最小抑菌浓度(MIC)。[结果]去甲肾上腺素对于大肠杆菌的促生长效果具有浓度依赖性,最佳促生长浓度为100μmol·L-1。7株携带质粒介导的喹诺酮类耐药基因的大肠杆菌中有4株(A、B、C、D)成功获得接合子,经去甲肾上腺素(100μmol·L-1)诱导后与受体菌的接合转移率与去甲肾上腺素未诱导组相比均升高,其中A、B、C 3株供体菌极显著升高(P<0.01)。所有接合子均扩增到恩诺沙星3种耐药基因:aac(6')-lb-cr、oqx AB、qnr S。接合子菌株对6种抗菌药物的MIC值与大部分供体菌株相同。[结论]去甲肾上腺素对于大肠杆菌的促生长效果具有浓度依赖性,且提高了大肠杆菌分离株耐药质粒体外接合转移率。提示:动物养殖生产中应加强应激管理,以减少细菌耐药基因水平传播的发生。
译  名：
Influence of norepinephrine on resistant plasmids conjugational transfer between Escherichia coli
作  者：
WENG Mengwei;JI Xu;ZHENG Weijiang;YAO Wen;College of Animal Science and Technology,Nanjing Agricultural University;
关键词：
norepinephrine;;Escherichia coli;;drug resistance;;conjugational transfer rate
摘  要：
[Objectives]The aim at this paper was to evaluate the potential effects of acute stress factors on the level of the horizontal transfer of bacterial resistant genes in the animal production. [Methods]Two isolates from weaned Xiang pigs were selected as test strains.The optimal growth promotion concentration of norepinepheine( NE) for Escherichia coli was determined by measuring the growth curves of the D600. Four isolates( A,B,C,D) from weaned Xiang pigs were used to carry plasmid-mediated enrofloxacin resistant genes( aac( 6')-lb-cr,oqx AB,qnr S,the transfer rate of the conjugation between the donors( induced by NE or not) and the recipient( E. coli J53) were calculated in filter mating. The minimum inhibitory concentrations( MIC) of the transconjugants,donor strains and the recipient strain to 6 antibiotics were determined by the Kirby·Bauer test and the agar dilution method. The plasmid-mediated enrofloxacin resistant genes of the transconjugants,donor strains and the recipient strain were detected by PCR. [Results]The E. coli growth was promoted by NE dose-dependently,and the optimal growth promotion concentration was determined by the administration of100 μmol·L-1NE. The transfer rates of the conjugation between three donors( A,B,C) induced by NE( 100 μmol·L-1) and the recipient were significantly higher than those of control groups( the donors without exposure to NE)( P<0.01). Three plasmid-mediated enrofloxacin resistant genes( aac( 6')-lb-cr,oqx AB,qnr S) were detected in all transconjugants. The MIC of the transconjugants to 6 antibiotics were the same as those of the donor strains. [Conclusions]NE increased the transfer frequency of resistant plasmids of isolates in vitro,in addition,the E. coli growth was promoted by NE dose-dependently. Suggestion that the management of stress should be strengthened in the animal production and breeding in order to reduce the horizontal transfer of bacterial resistant genes.

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