当前位置: 首页 > 文章 > 除草剂室内高通量筛选方法研究 湖北农业科学 2011,50 (24) 5119-5122
Position: Home > Articles > Study on High Throughput Screening of Herbicide Hubei Agricultural Sciences 2011,50 (24) 5119-5122

除草剂室内高通量筛选方法研究

作  者:
张志刚;杨自文;王开梅;张亚妮;张遵霞
单  位:
湖北省生物农药工程研究中心;湖北省生物农药工程研究中心湖北省农业科技创新中心生物农药分中心;湖北省生物农药工程研究中心/湖北省农业科技创新中心生物农药分中心
关键词:
高通量筛选;除草活性;种子处理;抗真菌剂
摘  要:
以狗牙根为材料,对除草剂筛选的限制因素进行了研究。结果表明,水分流失和真菌污染是引起筛选结果不稳定的主要因素;运用多种规格的组织培养板和雾化补水可以控制水分流失对筛选的不利影响;添加抗真菌剂和对种子进行杀菌处理可以消除真菌污染对靶标的侵害和延长试验的可观察时间。进一步的筛选试验结果表明,运用以上方法能够稳定、高效地对不同来源的样品进行筛选。
译  名:
Study on High Throughput Screening of Herbicide
作  者:
ZHANG Zhi-gang,YANG Zi-wen,WANG Kai-mei,ZHANG Ya-ni,ZHANG Zun-xia(Hubei Biopesticide Engineering Research Center/The Branch Center of Biopesticide,Hubei Agricultural Scientific and Technological Innovation Center,Wuhan 430064,China)
关键词:
high throughput screening;herbicidal activity;seeds treatment;fungicide.
摘  要:
In order to optimize the high throughput screening of herbicide,various factors that affect the growth of grass in testing plates were studied.The results showed that the key factors were the loss of water and fungal infection.So a screening model was advanced to break the series of limiting factors.The loss of water had been controlled by the application of different testing plates and addition of water by the atomizer;by the application of selective fungicide and seeds treatment,the problem of fungal infection had been solved and the period of testing had been lengthened.The screening method had been tested and verified with three standards and about 1280 samples from different sources.The results showed that it is a stable and highly efficient method for the screening of herbicide activity of samples from different sources.

相似文章

计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊