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当前位置: 首页 > 文章 > 宣和猪PRKAG3基因外显子5多态性及其与肉质性状的关联 云南农业大学学报(自然科学) 2020 (1) 26-32
Position: Home > Articles > Polymorphisms of the Exon 5 in PRKAG3 Gene and Its Association with Meat Quality Traits of Xuanhe Pigs Journal of Yunnan Agricultural University(Natural Science) 2020 (1) 26-32

宣和猪PRKAG3基因外显子5多态性及其与肉质性状的关联

作  者：
胡伟;董新星;张克忠;何家书;严达伟;李海莲;李明丽;鲁绍雄
单  位：
关键词：
宣和猪;PRKAG3基因;外显子5;单核苷酸多态性;肉质性状
摘  要：
【目的】揭示宣和猪PRKAG3基因外显子5多态性及其与肉质性状的关联。【方法】采用PCR产物直接测序法检测了120头宣和猪PRKAG3基因外显子5区域的SNP位点,借助最小二乘模型分析了各位点不同基因型及合并基因型对6个肉质性状的影响。【结果】宣和猪PRKAG3基因外显子5区域检出3个SNP位点,包括2个同义突变位点(T579C、T580C)和1个错义突变位点(G595A),分别以TT、TT、GA基因型和T、T、G等位基因的频率最高,且都处于Hardy-Weinberg平衡状态。T579C、T580C位点的TT和TC基因型可显著提高大理石纹(P<0.05)和降低失水率(P<0.01),分别呈显著的加性效应及加性—显性效应(P<0.05或P<0.01);G595A位点的AA基因型失水率最低(P<0.05)、熟肉率最高(P<0.05),呈显著的加性效应(P<0.05);3个位点的合并基因型TTTTGG、TCTCGG和TTTTAA分别具有最高的大理石纹、pH1和最低的失水率(P<0.05或P<0.01),CCCCGG基因型的大理石纹和pH1最低、失水率最高(P<0.05或P<0.01)。【结论】研究结果进一步确认宣和猪PRKAG3基因外显子5多态性与肌肉大理石纹、pH值和失水率显著关联,T579C、T580C位点的CC基因型和G595A位点的GG基因型具有协同降低猪肉品质的效应。
译  名：
Polymorphisms of the Exon 5 in PRKAG3 Gene and Its Association with Meat Quality Traits of Xuanhe Pigs
作  者：
HU Wei;DONG Xinxing;ZHANG Kezhong;HE Jiashu;YAN Dawei;LI Hailian;LI Mingli;LU Shaoxiong;Faculty of Animal Science and Technology,Yunnan Agricultural University;Yunnan Provincial Key Laboratory of Animal Nutrition and Feed;Xuanwei Livestock and Poultry Improvement Station;
关键词：
Xuanhe pig;;protein kinase adenosine monophosphate gamma 3 subunit(PRKAG3);;exon 5;;single nucleotide polymorphism(SNP);;meat quality trait
摘  要：
[Purpose]The aim of the present study was to investigate the polymorphisms of the exon 5 in protein kinase adenosine monophosphate gamma 3 subunit(PRKAG3) gene and its association with meat quality traits in Xuanhe pigs.[Method]The single nucleotide polymorphisms(SNPs) within the exon 5 of PRKAG3 gene were detected by PCR product directed sequencing in 120 Xuanhe pigs, the effects of single-locus and multiple-loci combined genotypes of the identified SNPs on 6 meat quality traits were analyzed by using least square models.[Result]Three SNPs were identified within the exon 5 of PRKAG3 gene, which included 2 synonymous mutations(T579 C,T580 C) and 1 missense mutation(G595 A), which were all in Hardy-Weinberg equilibrium. For the loci T579 C, T580 C and G595 A, the highest frequency was presented for the genotypes TT, TT, GA and the alleles T, T, G, respectively. The genotypes TT and TC of T579 C and T580 C could significantly increase the marbling score(MS)(P<0.05) and decrease the water loss rate(WLR)(P<0.01),which presented as significant additive and additive-dominance effects(P<0.05 or P<0.01); pigs with genotype AA on locus G595 A had the lowest WLR and the highest cooked rate(P<0.05), which presented as significant additive effects(P<0.05); the three-loci combined genotypes TTTTGG,TCTCGG and TTTTAA were the highest on MS and pH1, and lowest on WLR(P<0.05 or P<0.01),respectively; but the genotype CCCCGG was the lowest on MS and pH1, and highest on WLR(P<0.05 or P<0.01).[Conclusion]The present results further confirmed the polymorphisms in the exon 5 of PRKAG3 gene were significantly associated with the pork MS, pH and WLR in Xuanhe pigs, which the synergistic effects were presented for the genotype CC of T579 C/T580 C and the genotype GG of G595 A on decreasing the meat quality.

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