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当前位置: 首页 > 文章 > 宣和猪PLIN2基因5′-UTR和3′-UTR多态性及其与生长性状的关联分析 云南农业大学学报(自然科学) 2020 (1) 68-74
Position: Home > Articles > Polymorphisms of 5′-UTR and 3′-UTR of PLIN2 Gene and Their Associations with Growth Traits in Xuanhe Pig Journal of Yunnan Agricultural University(Natural Science) 2020 (1) 68-74

宣和猪PLIN2基因5′-UTR和3′-UTR多态性及其与生长性状的关联分析

作  者：
李俄然;曾韦植;王孝义;鲁绍雄;胡伟;何家书;严达伟;李明丽
单  位：
关键词：
宣和猪;PLIN2基因;非翻译区;单核苷酸多态性;生长性状
摘  要：
【目的】揭示PLIN2基因对宣和猪生长性状的影响。【方法】以357头宣和猪为试验材料,采用PCR产物直接测序法检测了PLIN2基因的5′-UTR和3′-UTR多态性,分析了各SNP位点不同基因型的生长性状差异。【结果】在5′-UTR检测到2个SNP位点(G158A、T491G)、3′-UTR检出1个SNP位点(G5689A),上述3个SNP位点分别以GG、TT和GG基因型频率最高,G、T和G为优势等位基因;除G158A位点未达到Hardy-Weinberg平衡状态(P<0.01)外,其余2个突变位点均处于平衡状态(P>0.05),且杂合度较高、遗传多样性较为丰富。在6月龄体重、4—6月龄日增重和70日龄—6月龄日增重上,G158A位点的GG型、T491G位点的TT型和G5689A位点的GG型均显著高于同位点其他基因型(P<0.01或P<0.05);且3个SNP位点不同单倍型组合对生长性状的影响呈现了明显的协同作用,即GTG/GTG组合的6月龄体重、4—6月龄日增重和70日龄—6月龄日增重最高(P<0.01或P<0.05)。【结论】研究结果从PLIN2基因多态性角度印证了宣和猪新品种选育工作的有效性,初步证实PLIN2基因与宣和猪生长性状间存在显著关联。
译  名：
Polymorphisms of 5′-UTR and 3′-UTR of PLIN2 Gene and Their Associations with Growth Traits in Xuanhe Pig
作  者：
LI Eran;ZENG Weizhi;WANG Xiaoyi;LU Shaoxiong;HU Wei;HE Jiashu;YAN Dawei;LI Mingli;Faculty of Animal Science and Technology,Yunnan Agricultural University;Yunnan Provincial Key Laboratory of Animal Nutrition and Feed;Animal Husbandry and Veterinary Bureau of Xuanwei City;
关键词：
Xuanhe pig;;PLIN2 gene;;untranslated region(UTR);;single nucleotide polymorphism(SNP);;growth trait
摘  要：
[Purpose]The aim of the present study was to reveal the effect of PLIN2 gene polymorphisms on the growth traits of Xuanhe pigs.[Method]A sample of 357 Xuanhe pigs were used to screen the polymorphisms of 5′-untranslated region(UTR) and 3′-UTR of PLIN2 gene by PCR products directed sequencing, and the differences of growth traits of different genotypes at each single nucleotide polymorphism(SNP) locus were analyzed.[Result]Three SNPs were identified at 5′-UTR and 3′-UTR of PLIN2 gene, which two(G158 A, T491 G) at the 5′-UTR and one(G5689 A) at the3′-UTR. For the the three SNPs, the genotype frequencies of GG, TT and GG genotypes were the highest, and the alleles G, T and G were dominant alleles, respectively; the loci T491 G and G5689 A were medium polymorphic and abundant in genetic diversity, and were in equilibrium(P>0.05), except the locus G158 A. The genotype GG of G158 A, TT of T491 G and GG of G5689 A were significantly higher than the other genotypes of the same locus on the body weight(BW) at 6 months, average daily gain(ADG) of 4 to 6 months and ADG of 70 days to 6 months(P<0.01 or P<0.05). In addition, there were obvious synergistic effects on the growth traits for different haplotype combinations of the three SNP loci, and the GTG/GTG was the highest among the all combinations on the BW at 6 months, ADG of 4 to 6 months and ADG of 70 days to 6 months(P<0.01 or P<0.05).[Conclusion]The present results further confirmed the effectiveness of the breeding of the new breed Xuanhe pig from the polymorphisms of PLIN2 gene, and preliminarily confirmed the significant association of PLIN2 gene with growth traits in Xuanhe pigs.

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