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Position: Home > Articles > Analyzing of the VP3 Gene Sequence of Goose Parvovirus H Strain China Animal Husbandry & Veterinary Medicine 2010,37 (10) 155-158

小鹅瘟病毒H株VP3基因序列分析

作  者:
王彬;孙德君;柴华;王丹娜;潘兴广
单  位:
哈药集团生物疫苗有限公司研发中心
关键词:
小鹅瘟病毒;VP3基因;序列分析
摘  要:
试验利用PCR技术从自行分离的GPV基因组DNA中扩增出病毒衣壳蛋白VP3完整基因片段,并与pMD18-T载体连接,转化大肠杆菌DH 5α感受态细胞,提取重组质粒经PCR和酶切鉴定后,对插入片段进行序列测定及分析。结果表明,VP3基因全长1605 bp,编码534个氨基酸;分子生物学分析表明所分离到的病毒为强毒株。
译  名:
Analyzing of the VP3 Gene Sequence of Goose Parvovirus H Strain
作  者:
WANG Bin,SUN De-jun,CHAI Hua,WANG Dan-na,PAN Xing-guang(Harbin Phermaceutical Group Bio-vaccine Co.Ltd.,R and D Center,Harbin 150069,China)
关键词:
GPV;VP3 gene;sequence analysis
摘  要:
VP3 gene of goose parvovirus was amplified from the DNA by polymerase chain reaction(PCR),the VP3 gene was cloned into pMD18-T vector.The recombinant plasmid was indentified with restriction endonuclease analysis and PCR,and then sequenced.The result of sequence analysis showed that VP3 gene had 1605 bp and included a complete open reading frame which encoding a protein of 534 amino acids,and the analysis of molecular biology showed that the virus was virulent strain.

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