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Position: Home > Articles > Cloning and Characterization of a Salt Tolerance-Associated Gene Encoding Trehalose-6-Phosphate Synthase in Sweetpotato Journal of Integrative Agriculture 2014,13 (8)

Cloning and Characterization of a Salt Tolerance-Associated Gene Encoding Trehalose-6-Phosphate Synthase in Sweetpotato

作者：

Jiang Tao;Zhai Hong;Wang Fei-bing;Zhou Hua-nan;Si Zeng-zhi;He Shao-zhen;Liu Qing-chang

单位：

China Agr Univ, Coll Agr & Biotechnol, Lab Crop Heterosis & Utilizat, Beijing Key Lab Crop Genet Improvement,Minist Edu, Beijing 100193, Peoples R China

关键词：

cloning;IbTPS gene;Ipomoea batatas (L.) Lam.;salt tolerance

摘要：

Trehalose plays an important role in metabolic regulation and abiotic stress tolerance in a variety of organisms. In plants, its biosynthesis is catalyzed by two key enzymes: trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP). In the present study, a TPS gene, named IbTPS, was first isolated from sweetpotato (Ipomoea batatas (L.) Lam.) cv. Lushu 3 by rapid amplification of cDNA ends (RACE). The open reading frame (ORF) contained 2 580 nucleotides encoding 859 amino acids with a molecular weight of 97.433 k.Da and an isoelectric point (pI) of 5.7. The deduced amino acid sequence showed high identifies with TPS of other plants. Real-time quantitative PCR analysis revealed that the expression level of IbTPS gene was significantly higher in stems of Lushu 3 than in its leaves and roots. Subcellular localization analysis in onion epidermal cells indicated that IbTPS gene was located in the nucleus. Transgenic tobacco (cv. Wisconsin 38) plants over-expressing IbTPS gene exhibited significantly higher salt tolerance compared with the control plant. Trehalose and proline content was found to be significantly more accumulated in transgenic tobacco plants than in the wild-type and several stress tolerance related genes were up-regulated. These results suggest that IbTPS gene may enhance salt tolerance of plants by increasing the amount of treahalose and proline and regulating the expression of stress tolerance related genes.

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Cloning and Characterization of a Salt Tolerance-Associated Gene Encoding Trehalose-6-Phosphate Synthase in Sweetpotato

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