作 者：
房希碧;贾泓瑶;张昕;石惠;郝林琳;刘松财;田来明
单 位：
吉林大学动物科学学院;吉林大学白求恩第一医院
关键词：
IGFBP-6基因;西藏小型猪;实时荧光定量PCR;mRNA表达水平
摘 要：
采用实时荧光定量PCR(Real Time PCR)方法,对不同生长阶段的西藏小型猪和军牧一号白猪的肝脏、脾脏、肾脏及肌肉组织中胰岛素样生长因子结合蛋白6(IGFBP-6)基因mRNA表达水平进行检测,并利用t检验,对相同生长阶段的各组织2个品种猪之间的表达水平进行比较分析。结果表明:西藏小型猪与军牧一号白猪IGFBP-6基因均在肌肉组织中表达量最高,而在肝组织中表达量最低。IGFBP-6基因在1日龄肾脏中,西藏小型猪表达水平高于军牧一号白猪,差异显著(P<0.05);在脾脏组织中,西藏小型猪表达水平低于军牧一号白猪,表达水平差异极显著(P<0.01)。其他生长阶段和组织中,2个品种猪IGFBP-6基因表达水平差异均不显著(P>0.05)。
译 名：
Differential Expression of IGFBP-6 Gene between the Tibetan Mini-Pig and the Junmu No. 1 White Pig
作 者：
FANG Xi-bi;JIA Hong-yao;ZHANG Xin;SHI Hui;HAO Lin-lin;LIU Song-cai;TIAN Lai-ming;College of Animal Sciences, Jilin University;The First Hospital of Jilin University;Changchun City Academy of Agricultural Sciences;
关键词：
IGFBP-6 gene;;Tibetan mini-pig;;real time PCR;;mRNA expression level
摘 要：
Insulin-like growth factorbinding protein 6(IGFBP-6),amember of insulin-like growth factor binding protein family(IGFBPs), was given extensive attention by researchers because ithashigh affinity with IGF-2 compared with IGF-1.However,the function of IGFBP-6 gene in pig growth and development is unclear. In this research,the mRNA expression levels of IGFBP-6 were tested by real time PCRin liver, kidney, spleen and muscle of Tibetan mini-pig and Junmu No. 1 White pig, and Ttest was used to analyze the expression levels between the two breeds at the same growth stage. The result showed that IGFBP-6 gene was expressed widely in pigs. The expression level of IGFBP-6 gene in muscle was the highest,while in the liver it was the lowest. IGFBP-6 mRNA expression level in kidney was significantly different between Tibetan mini-pigs(1d) and Junmu No. 1 White pigs(1 d)(P< 0.05).The expression level in spleen was extremely significantly lowerin Tibetanmini-pigs(1 d)than JunmuNo.1 White pigs(1 d)(P< 0.01). But IGFBP-6 mRNA expression in other tissues showed no significant difference among different breeds.This research will support function study of IGFBP-6 gene atthe molecular level, and the results may provide useful data for furtherbasic research to explore the mechanism of dwarfismin these pigs.
