作 者：
王玉英;王琴;李枝林;魏全涛;商正蕊;凌青
单 位：
云南农业职业技术学院;云南农业大学园林园艺学院
摘 要：
以兰属花卉虎雪兰组培原球茎为试材,采用玻璃化超低温法对兰花病毒脱除进行了研究,以期为虎雪兰玻璃化超低温法脱毒体系的建立提供参考依据。结果表明:在蔗糖浓度0.5 mol·L~(-1)预培养4 d,然后在蔗糖0.6 mol·L~(-1)加载液冰上处理50 min,之后转入PVS2溶液冰上玻璃化处理120 min,再液氮冷冻40 min,37℃水浴解冻3 min,最后卸载液(1/2MS+1.2 mol·L~(-1)蔗糖)卸载20 min,待恢复培养后,原球茎成活率可达到65%以上,随机检测不同处理样品的脱毒率可达97%。
译 名：
Study on Cymbidium tracyanum var. huanghua×Cymbidium mastersii on Virus Elimination Using Vitrification-cryopreservation Technology
作 者：
WANG Yuying;WANG Qin;LI Zhilin;WEI Quantao;SHANG Zhengrui;LING Qing;College of Garden and Horticulture,Yunnan Agricultural University;Yunnan Agricultural Vocational and Technical College;
关键词：
Cymbidium tracyanum var.huanghua×Cymbidium mastersii;;protocorm;;vitrification;;cryopreservation;;virus elimination
摘 要：
The original bulbs of the Cymbidium tracyanum var.huanghua×Cymbidium mastersii tissue culture were used as the test material.The vitrification ultra-low temperature method was used to study the removal of Cymbidium virus.It would provide reference for virus elimination technology of orchids.This experiment was a study about virus-elimicaton of orchids using vitrification-cryopreservation technology,and the tissue culture protocorm of Cymbidium tracyanum var.huanghua×Cymbidium mastersii was used as the experiment material.The results showed that pre-culture for 4 days in 0.5 mol·L~(-1)sucrose concentration,then treat 50 minutes in loading liquid that with sucrose concentration of 0.6 mol·L~(-1) on ice,the next step was shift into PVS-2 liquid and vitrification treatment 120 minutes on ice,then freeze 40 minutes in liquid nitrogen,after that thaw 3 minutes in 37 ℃ centigrade for 3 minutes,finally load-off 20 minutes in unloading liquid(1/2 MS+1.2 mol·L~(-1) sucrose).After resuming culture,the survive rate of protocorm could reach more than 65%,and the detoxification rate could reach 97% by random detection of different samples.
